Isocitrate Dehydrogenase Protein (IDH) (DYKDDDDK Tag)
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- Target Alle Isocitrate Dehydrogenase (IDH) Proteine anzeigen
- Isocitrate Dehydrogenase (IDH)
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- Insect cells (Sf9)
- Aufreinigungstag / Konjugat
- Dieses Isocitrate Dehydrogenase Protein ist gelabelt mit DYKDDDDK Tag.
- Applikation
- Antibody Production (AbP), Standard (STD)
- Produktmerkmale
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- Recombinant human Isocitrate dehydrogenase / IDH (C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- Reinheit
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product IDH Protein
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Kommentare
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The tag is located at the C-terminal.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 50 μg/mL
- Buffer
- 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Isocitrate Dehydrogenase (IDH)
- Andere Bezeichnung
- Isocitrate Dehydrogenase,idh (IDH Produkte)
- Synonyme
- CG7176 Protein, CT22171 Protein, Dmel\\CG7176 Protein, IDH Protein, IDH-NADP Protein, Idh-NADP Protein, idh Protein, isocitrate dehydrogenase Protein, l(3)L3852 Protein, F20D21.16 Protein, F20D21_16 Protein, SMU.672 Protein, icd Protein, Isocitrate dehydrogenase Protein, isocitrate dehydrogenase Protein, isocitrate dehydrogenase, NADP-dependent Protein, Monomeric isocitrate dehydrogenase Protein, ankyrin repeat domain 26-like Protein, Idh Protein, ICDH Protein, idh Protein, icd Protein, SSO_RS10660 Protein, JK_RS08640 Protein, LOC618625 Protein
- Hintergrund
- Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
- Molekulargewicht
- 47 kDa
- NCBI Accession
- NP_005887
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