CCL18
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, AbP, STD, PI
Active
CCL18
(Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Spezies
Human
Quelle
Escherichia coli (E. coli)
Applikation
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
Spezifität
Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
Recombinant human MIP4 / CCL18 protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Reinheit
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Endotoxin-Niveau
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Assay #1: Determined by its ability to chemoattract human T lymphocytes using a concentration of 1.0-10.0 ng/ml. Assay #2: Determined by its ability to chemoattract human iDCs using a concentration of 1.0-10.0 ng/ml.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Handhabung
Resuspend the protein in the desired concentration in proper buffer
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Target
CCL18
(Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses.