PAM Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
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- Target Alle PAM Proteine anzeigen
- PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- Transcript Variant 3
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses PAM Protein ist gelabelt mit Myc-DYKDDDDK Tag.
- Applikation
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Spezifität
- Optimal preservation of protein structure, post-translational modifications and functions.
- Produktmerkmale
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- Recombinant human PAM (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Reinheit
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- The specific activity of PAM was determined by measuring the product D-Tyr-Val-NH2 formation from a conversion of D-Tyr-Val-Gly. The reaction was carried out at 37C for 60min in the buffer containing 50 mM MES, pH6.0, 1 μM CuCl2, 2000 units/ml of Catalase, 5 mM L-ascorbic acid, and 50 uM of D-Tyr-Val-Gly as the substrate
- Top Product
- Discover our top product PAM Protein
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Kommentare
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The tag is located at the C-terminal.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
- Andere Bezeichnung
- Pam (PAM Produkte)
- Synonyme
- PAL Protein, PHM Protein, pal Protein, phm Protein, pamb Protein, CG3832 Protein, Dmel\\CG3832 Protein, dPHM Protein, AE-I Protein, PAL-A Protein, PHM-A Protein, pama Protein, si:dkeyp-35f12.2 Protein, peptidylglycine alpha-amidating monooxygenase Protein, peptidylglycine alpha-amidating monooxygenase S homeolog Protein, Peptidylglycine-alpha-hydroxylating monooxygenase Protein, peptidylglycine alpha-amidating monooxygenase L homeolog Protein, PAM Protein, Pam Protein, pam.S Protein, CHLREDRAFT_150435 Protein, sce7070 Protein, pam Protein, Phm Protein, pam.L Protein
- Hintergrund
- Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. [UniProtKB/Swiss-Prot Function]
- Molekulargewicht
- 94.2 kDa
- NCBI Accession
- NP_620176
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