IRF3
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
Handhabung
Aliquot to avoid repeated freezing and thawing.
Lagerung
-80 °C
Informationen zur Lagerung
Best use within three months from the date of receipt of this protein.
Pourcelot, Zemirli, Silva Da Costa, Loyant, Garcin, Vitour, Munitic, Vazquez, Arnoult: "The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing." in: BMC biology, Vol. 14, pp. 69, (2016) (PubMed).
Zhao, Tian, Edeh, Brasier: "Quantitation of the dynamic profiles of the innate immune response using multiplex selected reaction monitoring-mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 6, pp. 1513-29, (2013) (PubMed).
Wang, Li, Dorf: "NEMO binds ubiquitinated TANK-binding kinase 1 (TBK1) to regulate innate immune responses to RNA viruses." in: PLoS ONE, Vol. 7, Issue 9, pp. e43756, (2012) (PubMed).
Siu, Kok, Ng, Poon, Yuen, Zheng, Jin: "Severe acute respiratory syndrome coronavirus M protein inhibits type I interferon production by impeding the formation of TRAF3.TANK.TBK1/IKKepsilon complex." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16202-9, (2009) (PubMed).