SH3RF3 Protein (AA 1-882) (Strep Tag)
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- Target Alle SH3RF3 Produkte
- SH3RF3 (SH3 Domain Containing Ring Finger 3 (SH3RF3))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-882
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Spezies
- Human
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses SH3RF3 Protein ist gelabelt mit Strep Tag.
- Applikation
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- Sequenz
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MLLGASWLCA SKAAAAAAQS EGDEDRPGER RRRRAAATAA GAGEDMDESS LLDLLECSVC LERLDTTAKV LPCQHTFCRR CLESIVCSRH ELRCPECRIL VGCGVDELPA NILLVRLLDG IRQRPRAGTS PGGSPPARPI PGQSAAPTLA GGGGGAAGST PGSPVFLSAA AGSTAGSLRE LATSRTAPAA KNPCLLPYGK ALYSYEGKEP GDLKFNKGDI IVLRRKVDEQ WYHGELHGTQ GFLPASYIQC IQPLPHAPPQ GKALYDFEMK DKDQDKDCLT FTKDEILTVL RRVDENWAEG MLGDKIGIFP LLYVELNDSA KQLIEMDKPC PAAASSCNAS LPSDSGAVAS VAPSPTLSSS GAVSAFQRRV DGKKNTKKRH SFTALSVTHR SSQAASHRHS MEISAPVLIS SSDPRAAARI GDLAHLSCAA PTQDVSSSAG STPTAVPRAA SVSGEQGTPP KVQLPLNVYL ALYAYKPQKS DELELHKGEM YRVLEKCQDG WFKGASLRTG VSGVFPGNYV TPVSRVPAGG AGPPRNNVVG GSPLAKGITT TMHPGSGSLS SLATATRPAL PITTPQAHAQ HPTASPPTGS CLRHSAQPTA SQARSTISTA AHSAAQAQDR PTATVSPLRT QNSPSRLPAT SLRPHSVVSP QHSHQPPVQM CPRPAIPLTS AASAITPPNV SAANLNGEAG GGPIGVLSTS SPTNTGCKLD EKKSEKKEKK SGLLKLLAGA STKKKSRSPP SVSPTHDPQV AVDALLQGAV GPEVSSLSIH GRAGSCPIES EMQGAMGMEP LHRKAGSLDL NFTSPSRQAP LSMAAIRPEP KLLPRERYRV VVSYPPQSEA EIELKEGDIV FVHKKREDGW YKGTLQRNGR TGLFPGSFVE SF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- SH3RF3 (SH3 Domain Containing Ring Finger 3 (SH3RF3))
- Andere Bezeichnung
- SH3RF3 (SH3RF3 Produkte)
- Synonyme
- Sh3md4 Protein, SH3MD4 Protein, SH3RF1 Protein, MGC131299 Protein, POSH2 Protein, 4831416G18Rik Protein, Igfbp5-ip Protein, Sh3rf31 Protein, SH3 domain containing ring finger 3 Protein, SH3 domain containing ring finger 3 L homeolog Protein, Sh3rf3 Protein, SH3RF3 Protein, sh3rf3.L Protein, sh3rf3 Protein
- Hintergrund
- E3 ubiquitin-protein ligase SH3RF3 (EC 2.3.2.27) (Plenty of SH3s 2) (SH3 domain-containing RING finger protein 3) (SH3 multiple domains protein 4),FUNCTION: Has E3 ubiquitin-protein ligase activity. {ECO:0000269|PubMed:20696164}.
- Molekulargewicht
- 92.8 kDa
- UniProt
- Q8TEJ3
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