PDHA1 Protein (AA 31-390) (His tag)
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- Target Alle PDHA1 Proteine anzeigen
- PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 31-390
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Spezies
- Human
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses PDHA1 Protein ist gelabelt mit His tag.
- Applikation
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequenz
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ANDATFEIKK CDLHRLEEGP PVTTVLTRED GLKYYRMMQT VRRMELKADQ LYKQKIIRGF CHLCDGQEAC CVGLEAGINP TDHLITAYRA HGFTFTRGLS VREILAELTG RKGGCAKGKG GSMHMYAKNF YGGNGIVGAQ VPLGAGIALA CKYNGKDEVC LTLYGDGAAN QGQIFEAYNM AALWKLPCIF ICENNRYGMG TSVERAAAST DYYKRGDFIP GLRVDGMDIL CVREATRFAA AYCRSGKGPI LMELQTYRYH GHSMSDPGVS YRTREEIQEV RSKSDPIMLL KDRMVNSNLA SVEELKEIDV EVRKEIEDAA QFATADPEPP LEELGYHIYS SDPPFEVRGA NQWIKFKSVS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PDHA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product PDHA1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))
- Andere Bezeichnung
- PDHA1 (PDHA1 Produkte)
- Synonyme
- PDHA Protein, PDHCE1A Protein, PHE1A Protein, Pdha-1 Protein, pdha Protein, phe1a Protein, pdhce1a Protein, im:6895726 Protein, pdha1 Protein, wu:fd18b01 Protein, wu:fo96f03 Protein, zgc:73271 Protein, zgc:86692 Protein, Pdha1 Protein, wu:fp73b04 Protein, zgc:92705 Protein, NI36_05250 Protein, pdha1-a Protein, pdha1-b Protein, pyruvate dehydrogenase E1 alpha 1 subunit Protein, pyruvate dehydrogenase E1 alpha 1 Protein, pyruvate dehydrogenase (lipoamide) alpha 1 Protein, pyruvate dehydrogenase (lipoamide) alpha 1 pseudogene Protein, 2-oxo acid dehydrogenase Protein, pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha Protein, pyruvate dehydrogenase alpha subunit protein Protein, pyruvate dehydrogenase (acetyl-transferring) protein, alpha subunit Protein, precursor of dehydrogenase pyruvate dehydrogenase E1 component alpha subunit Protein, pyruvate dehydrogenase Protein, pyruvate dehydrogenase E1 alpha 1 subunit a Protein, pyruvate dehydrogenase E1 alpha 1 subunit b Protein, pyruvate dehydrogenase E1 component subunit alpha Protein, pyruvate dehydrogenase (lipoamide) alpha 1 S homeolog Protein, PDHA1 Protein, Pdha1 Protein, pdha1 Protein, LOC719277 Protein, VNG_RS07485 Protein, RR_RS04695 Protein, pdhA1 Protein, pdha1a Protein, pdha1b Protein, NI36_RS05240 Protein, pdha1.S Protein
- Hintergrund
- The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. {ECO:0000269|PubMed:19081061, ECO:0000269|PubMed:7782287}.
- Molekulargewicht
- 41.0 kDa Including tag.
- UniProt
- P08559
- Pathways
- Warburg Effekt
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