alpha 2 Antiplasmin Protein (AA 40-491) (His tag)
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- Target Alle alpha 2 Antiplasmin (SERPINF2) Proteine anzeigen
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 40-491
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses alpha 2 Antiplasmin Protein ist gelabelt mit His tag.
- Applikation
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequenz
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NQEQVSPLTL LKLGNQEPGG QTALKSPPGV CSRDPTPEQT HRLARAMMAF TADLFSLVAQ TSTCPNLILS PLSVALALSH LALGAQNHTL QRLQQVLHAG SGPCLPHLLS RLCQDLGPGA FRLAARMYLQ KGFPIKEDFL EQSEQLFGAK PVSLTGKQED DLANINQWVK EATEGKIQEF LSGLPEDTVL LLLNAIHFQG FWRNKFDPSL TQRDSFHLDE QFTVPVEMMQ ARTYPLRWFL LEQPEIQVAH FPFKNNMSFV VLVPTHFEWN VSQVLANLSW DTLHPPLVWE RPTKVRLPKL YLKHQMDLVA TLSQLGLQEL FQAPDLRGIS EQSLVVSGVQ HQSTLELSEV GVEAAAATSI AMSRMSLSSF SVNRPFLFFI FEDTTGLPLF VGSVRNPNPS APRELKEQQD SPGNKDFLQS LKGFPRGDKL FGPDLKLVPP MEEDYPQFGS PK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SERPINF2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product SERPINF2 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
- Andere Bezeichnung
- SERPINF2 (SERPINF2 Produkte)
- Synonyme
- A2AP Protein, AAP Protein, ALPHA-2-PI Protein, API Protein, PLI Protein, serpin Protein, AI747498 Protein, Pli Protein, Serpimf2 Protein, serpinf2 Protein, serpinf2l Protein, wu:fd57d08 Protein, Dmrs91 Protein, Pedf Protein, serpin family F member 2 Protein, serpin family F member 1 Protein, serine (or cysteine) peptidase inhibitor, clade F, member 2 Protein, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a Protein, SERPINF2 Protein, Serpinf2 Protein, SERPINF1 Protein, serpinf2a Protein, Serpinf1 Protein
- Hintergrund
- Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. {ECO:0000269|PubMed:15853774}.
- Molekulargewicht
- 51.4 kDa Including tag.
- UniProt
- P08697
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones
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