ZGPAT Protein (AA 1-531) (Strep Tag)
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- Target Alle ZGPAT Proteine anzeigen
- ZGPAT (Zinc Finger, CCCH-Type with G Patch Domain (ZGPAT))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-531
- Spezies
- Human
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses ZGPAT Protein ist gelabelt mit Strep Tag.
- Applikation
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- Sequenz
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MDEESLESAL QTYRAQLQQV ELALGAGLDS SEQADLRQLQ GDLKELIELT EASLVSVRKS SLLAALDEER PGRQEDAEYQ AFREAITEAV EAPAAARGSG SETVPKAEAG PESAAGGQEE EEGEDEEELS GTKVSAPYYS SWGTLEYHNA MVVGTEEAED GSAGVRVLYL YPTHKSLKPC PFFLEGKCRF KENCRFSHGQ VVSLDELRPF QDPDLSSLQA GSACLAKHQD GLWHAARITD VDNGYYTVKF DSLLLREAVV EGDGILPPLR TEATESDSDS DGTGDSSYAR VVGSDAVDSA QSSALCPSLA VVGSDAVDSG TCSSAFAGWE VHTRGIGSRL LTKMGYEFGK GLGRHAEGRV EPIHAVVLPR GKSLDQCVET LQKQTRVGKA GTNKPPRCRG RGARPGGRPA PRNVFDFLNE KLQGQAPGAL EAGAAPAGRR SKDMYHASKS AKRALSLRLF QTEEKIERTQ RDIRSIQEAL ARNAGRHSVA SAQLQEKLAG AQRQLGQLRA QEAGLQQEQR KADTHKKMTE F
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ZGPAT (Zinc Finger, CCCH-Type with G Patch Domain (ZGPAT))
- Andere Bezeichnung
- ZGPAT (ZGPAT Produkte)
- Synonyme
- zc3h9 Protein, gpatc6 Protein, gpatch6 Protein, zc3hdc9 Protein, GPATC6 Protein, GPATCH6 Protein, KIAA1847 Protein, ZC3H9 Protein, ZC3HDC9 Protein, ZIP Protein, 1500006I01Rik Protein, BC021513 Protein, RGD1310801 Protein, zgc:63730 Protein, zinc finger CCCH-type and G-patch domain containing Protein, zinc finger, CCCH-type with G-patch domain L homeolog Protein, zinc finger, CCCH-type with G-patch domain Protein, zinc finger, CCCH-type with G patch domain Protein, ZGPAT Protein, zgpat.L Protein, zgpat Protein, Zgpat Protein
- Hintergrund
- Zinc finger CCCH-type with G patch domain-containing protein (G patch domain-containing protein 6) (Zinc finger CCCH domain-containing protein 9) (Zinc finger and G patch domain-containing protein),FUNCTION: Transcription repressor that specifically binds the 5'-GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negatively regulates expression of EGFR, a gene involved in cell proliferation, survival and migration. Its ability to repress genes of the EGFR pathway suggest it may act as a tumor suppressor. Able to suppress breast carcinogenesis. {ECO:0000269|PubMed:19644445}., FUNCTION: [Isoform 4]: Antagonizes the transcription repression by isoform 1 by competing for the binding of the NuRD complex. Does not bind DNA. {ECO:0000269|PubMed:19644445}.
- Molekulargewicht
- 57.4 kDa
- UniProt
- Q8N5A5
- Pathways
- EGFR Signaling Pathway
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