ERAP2 Protein (AA 1-960) (Strep Tag)
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- Target Alle ERAP2 Proteine anzeigen
- ERAP2 (Endoplasmic Reticulum Aminopeptidase 2 (ERAP2))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-960
- Spezies
- Human
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses ERAP2 Protein ist gelabelt mit Strep Tag.
- Applikation
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Sequenz
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MFHSSAMVNS HRKPMFNIHR GFYCLTAILP QICICSQFSV PSSYHFTEDP GAFPVATNGE RFPWQELRLP SVVIPLHYDL FVHPNLTSLD FVASEKIEVL VSNATQFIIL HSKDLEITNA TLQSEEDSRY MKPGKELKVL SYPAHEQIAL LVPEKLTPHL KYYVAMDFQA KLGDGFEGFY KSTYRTLGGE TRILAVTDFE PTQARMAFPC FDEPLFKANF SIKIRRESRH IALSNMPKVK TIELEGGLLE DHFETTVKMS TYLVAYIVCD FHSLSGFTSS GVKVSIYASP DKRNQTHYAL QASLKLLDFY EKYFDIYYPL SKLDLIAIPD FAPGAMENWG LITYRETSLL FDPKTSSASD KLWVTRVIAH ELAHQWFGNL VTMEWWNDIW LKEGFAKYME LIAVNATYPE LQFDDYFLNV CFEVITKDSL NSSRPISKPA ETPTQIQEMF DEVSYNKGAC ILNMLKDFLG EEKFQKGIIQ YLKKFSYRNA KNDDLWSSLS NSCLESDFTS GGVCHSDPKM TSNMLAFLGE NAEVKEMMTT WTLQKGIPLL VVKQDGCSLR LQQERFLQGV FQEDPEWRAL QERYLWHIPL TYSTSSSNVI HRHILKSKTD TLDLPEKTSW VKFNVDSNGY YIVHYEGHGW DQLITQLNQN HTLLRPKDRV GLIHDVFQLV GAGRLTLDKA LDMTYYLQHE TSSPALLEGL SYLESFYHMM DRRNISDISE NLKRYLLQYF KPVIDRQSWS DKGSVWDRML RSALLKLACD LNHAPCIQKA AELFSQWMES SGKLNIPTDV LKIVYSVGAQ TTAGWNYLLE QYELSMSSAE QNKILYALST SKHQEKLLKL IELGMEGKVI KTQNLAALLH AIARRPKGQQ LAWDFVRENW THLLKKFDLG SYDIRMIISG TTAHFSSKDK LQEVKLFFES LEAQGSHLDI FQTVLETITK NIKWLEKNLP TLRTWLMVNT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ERAP2 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ERAP2 (Endoplasmic Reticulum Aminopeptidase 2 (ERAP2))
- Andere Bezeichnung
- ERAP2 (ERAP2 Produkte)
- Synonyme
- L-RAP Protein, LRAP Protein, ERAP2 Protein, zgc:172163 Protein, endoplasmic reticulum aminopeptidase 2 Protein, ERAP2 Protein, erap2 Protein
- Hintergrund
- Endoplasmic reticulum aminopeptidase 2 (EC 3.4.11.-) (Leukocyte-derived arginine aminopeptidase) (L-RAP),FUNCTION: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys. {ECO:0000269|PubMed:12799365, ECO:0000269|PubMed:15908954, ECO:0000269|PubMed:16286653}.
- Molekulargewicht
- 110.5 kDa
- UniProt
- Q6P179
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