Adracalin Protein (AA 2-546) (His tag)
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- Target Alle Adracalin (AAAS) Proteine anzeigen
- Adracalin (AAAS) (Achalasia, Adrenocortical Insufficiency, Alacrimia (AAAS))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 2-546
- Spezies
- Maus
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses Adracalin Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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CSLGLFPPPP PRGQVTLYEH NNELVTGNSY ESPPPDFRGQ WINLPVLHLT KDPLKAPGRL DHGTRTAFIH HREQVWKRCI NVWHDVGLFG VLNEIANSEE EVFEWVKTAC SWALALCGRA SSLHGSLFPH LSLRSEDLIA EFAQVTNWSS CCLRVFAWHP HTNKFAVALL DDSIRVYNAN STIVPSLKHR LQRNVAALAW KPLSASVLAV ACQSCILIWT LDPTSLSTRP SSGCAQVLSH PGHTPVTSLA WAPNGGWLLS ASPVDAVILV WDVSTETCVP LPWFRGGGVT NLLWSPDGSK VLATTPSAVF RVWEAQMWTC EAWPTLSGRC QTGCWSPDGN RLLFTVLGEA LIYSLSFPER CGTGKGHVGG AKSATIVADL SETTIQTPDG EERLGGEAHS MVWDPSGERL AVLMKGNPQV QDGNPVILLF RTRNSPVFEL LPCGIIQGEP GAQAQLITFH PSFNKGALLS VCWSTGRITH IPLYFVNAQF PRFSPVLGRA QEPPAGGGGS IHEVPLFTET SPTSAPWDPL PGQSSAQPHS PHSHL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Aaas Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product AAAS Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- Adracalin (AAAS) (Achalasia, Adrenocortical Insufficiency, Alacrimia (AAAS))
- Andere Bezeichnung
- Aaas (AAAS Produkte)
- Synonyme
- zgc:85873 Protein, aladin Protein, Aladin Protein, AAA Protein, AAASb Protein, ADRACALA Protein, ADRACALIN Protein, ALADIN Protein, D030041N15Rik Protein, GL003 Protein, aladin WD repeat nucleoporin Protein, achalasia, adrenocortical insufficiency, alacrimia Protein, aladin WD repeat nucleoporin L homeolog Protein, Aaas Protein, aaas Protein, aaas.L Protein, AAAS Protein
- Hintergrund
- Plays a role in the normal development of the peripheral and central nervous system. {ECO:0000250}.
- Molekulargewicht
- 60.3 kDa Including tag.
- UniProt
- P58742
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