SOX13 Protein (AA 1-613) (Strep Tag)
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- Target Alle SOX13 Proteine anzeigen
- SOX13 (SRY (Sex Determining Region Y)-Box 13 (SOX13))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-613
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses SOX13 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MSMQSPVSVQ LAPDSASTMV NCTIKSEEKK EPCHEAPQGA APAVETQPGD PALASQDATN AKAPPQDCAS PESSGSPEPK RPAASEAASG SQERLDFNRN LQEVVPAIEK LLSSDWKERF LGRSNVEAKD VKGTQESLAE KELQLLVMIH QLSALRDQLL TAHSEQKNMA AMLFEKQQQQ MELARQQQEQ IAKQQQQLIQ QQHKINLLQQ QIQQVNMPYV MIPAFPPSHQ PLPVTPDSQL ALPIQPIPCK PVEYPLQLLH SPPAPVVKRS GVAAHHPLQE PPQPLNLTAK PKVPELPNTS SSPSLKMNSC GPRPASHGAP TRDLQSSPPS LPLGFLGEGD AVTKAIQDAR QLLHSHSGAL ENSPNTPFRK DLISLDSSPA KERLEESCVH PLEEAMLSCD MDGSRHFSES RNSSHIKRPM NAFMVWAKDE RRKILQAFPD MHNSSISKIL GSRWKSMTNQ EKQPYYEEQA RLSRQHLEKY PDYKYKPRPK RTCVVEGRRL RVGEYKALMR TRRQGARQSY TIPPQAGQAQ VSSDILFPRA AGLPLARPLV EHYDPQGLDP NMPVIINTCS LREEGEGTDD RHSVADGEMY RYSEDEDSEG DEKSDEELVV LTD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product SOX13 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- SOX13 (SRY (Sex Determining Region Y)-Box 13 (SOX13))
- Andere Bezeichnung
- Sox13 (SOX13 Produkte)
- Synonyme
- ICA12 Protein, Sox-13 Protein, AA407916 Protein, AW259412 Protein, AW540933 Protein, SRY-box 13 Protein, SRY (sex determining region Y)-box 13 Protein, SRY box 13 Protein, SOX13 Protein, Sox13 Protein
- Hintergrund
- Transcription factor SOX-13 (SRY (Sex determining region Y)-box 13) (mSox13),FUNCTION: Transcription factor that binds to DNA at the consensus sequence 5'-AACAAT-3' (PubMed:9524265, PubMed:9421502). Binds to the proximal promoter region of the myelin protein MPZ gene, and may thereby be involved in the differentiation of oligodendroglia in the developing spinal tube (PubMed:26525805). Binds to the gene promoter of MBP and acts as a transcriptional repressor (PubMed:26525805). Binds to and modifies the activity of TCF7/TCF1, thereby inhibiting transcription and modulates normal gamma-delta T-cell development and differentiation of IL17A expressing gamma-delta T-cells (PubMed:17218525, PubMed:23562159, PubMed:30413363). Required for the differentiation of Vgamma2-positive gamma-delta T-cells, a subset of IL17A expressing gamma-delta T-cells (PubMed:23562159). Regulates expression of BLK in the differentiation of IL17A expressing gamma-delta T-cells (PubMed:23562159). Promotes brown adipocyte differentiation (PubMed:27923061). Inhibitor of WNT signaling (By similarity). {ECO:0000250|UniProtKB:Q9UN79, ECO:0000269|PubMed:17218525, ECO:0000269|PubMed:23562159, ECO:0000269|PubMed:26525805, ECO:0000269|PubMed:27923061, ECO:0000269|PubMed:30413363, ECO:0000269|PubMed:9421502, ECO:0000269|PubMed:9524265}.
- Molekulargewicht
- 68.2 kDa
- UniProt
- Q04891
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