ARHGAP1 Protein (AA 1-439) (Strep Tag)
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- Target Alle ARHGAP1 Proteine anzeigen
- ARHGAP1 (rho GTPase Activating Protein 1 (ARHGAP1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-439
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses ARHGAP1 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MDPLSELQDD LTLDDTSQAL NQLKLASIDE KNWPSDEMPD FPKSDDSKSS SPEPVTHLKW DDPYYDIARH QIVEVAGDDK YGRKIIVFSA CRMPPSHQLD HSKLLGYLKH TLDQYVESDY TLLYLHHGLT SDNKPSLSWL RDAYREFDRK YKKNIKALYI VHPTMFIKTL LILFKPLISF KFGRKIFYVN YLSELSEHVK LEQLGIPRQV LKYDDFLKST QKSPATAPKP MPPRPPLPNQ QFGVSLQHLQ EKSPGQDPIP IVLRETVAYL QAHALTTEGI FRRSANTQVV REVQQKYNMG LPVDFDQYNE LHLPAVILKT FLRELPEPLL TFDLYPHVVG FLNIDESQRV EVTQQVLQTL PEENYQVLHF LTAFLVQISA HCDQNKMTNT NLAVVFGPNL LWAKDAAITL KAINPINTFT KFLLDHQGEL FPSTDAQGV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ARHGAP1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ARHGAP1 (rho GTPase Activating Protein 1 (ARHGAP1))
- Andere Bezeichnung
- Arhgap1 (ARHGAP1 Produkte)
- Synonyme
- CDC42GAP Protein, RHOGAP Protein, RHOGAP1 Protein, p50rhoGAP Protein, B230365D05Rik Protein, C76222 Protein, Cdc42GAP Protein, RP23-12H6.2 Protein, fk03b12 Protein, fk61c06 Protein, wu:cegs2891 Protein, wu:fk03b12 Protein, wu:fk61c06 Protein, zgc:110361 Protein, arhgap1 Protein, arhgap1-a Protein, arhgap1-b Protein, cdc42gap Protein, p50rhogap Protein, rhogap Protein, rhogap1 Protein, rhogap68f Protein, Rho GTPase activating protein 1 Protein, Rho GTPase activating protein 1 S homeolog Protein, ARHGAP1 Protein, Arhgap1 Protein, arhgap1 Protein, arhgap1.S Protein
- Hintergrund
- Rho GTPase-activating protein 1 (Rho-type GTPase-activating protein 1),FUNCTION: GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate (By similarity). {ECO:0000250}.
- Molekulargewicht
- 50.4 kDa
- UniProt
- Q5FWK3
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