CLCN1 Protein (AA 1-114) (His tag)
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- Target Alle CLCN1 Proteine anzeigen
- CLCN1 (Chloride Channel 1, Skeletal Muscle (CLCN1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-114
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Spezies
- Maus
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses CLCN1 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- Sequenz
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MERSQSQRHG GEQSWWGSAP QYQYMPFEHC TSYGLPSENG GLQHRPRKDM GPRHNAHPTQ IYGHQKEQYS YKAQDGGMPK KMGSSSTMDS LDEDHYSKCQ DCVHRLGRVL RRKL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Clcn1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product CLCN1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- CLCN1 (Chloride Channel 1, Skeletal Muscle (CLCN1))
- Andere Bezeichnung
- Clcn1 (CLCN1 Produkte)
- Synonyme
- CLCN1 Protein, si:dkey-14o18.5 Protein, Clc-1 Protein, Clc1 Protein, SMCC1 Protein, adr Protein, mto Protein, myotonia Protein, nmf355 Protein, SMCC Protein, CLC1 Protein, chloride voltage-gated channel 1 Protein, chloride channel, voltage-sensitive 1a Protein, chloride channel protein 1 Protein, chloride channel, voltage-sensitive 1 Protein, CLCN1 Protein, clcn1a Protein, LOC100550479 Protein, LOC703944 Protein, Clcn1 Protein
- Hintergrund
- Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. {ECO:0000250|UniProtKB:P35523}.
- Molekulargewicht
- 14.1 kDa Including tag.
- UniProt
- Q64347
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