SCFD1 Protein (AA 1-639) (Strep Tag)
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- Target Alle SCFD1 Produkte
- SCFD1 (Sec1 Family Domain Containing 1 (SCFD1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-639
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses SCFD1 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MVGSKMAATA SIRERQTVAL KRMLNFNVPH VKNSTGEPVW KVLIYDRFGQ DIISPLLSVK ELRDMGITLH LLLHSDRDPI PDVPAVYFVM PTEENIDRLC QDLRNQLYES YYLNFISAIS RSKLEDIANA ALAASAVTQV AKVFDQYLNF ITLEDDMFVL CNQNKELVSY RAINRPDITD TEMETVMDTI VDSLFCFFVT LGAVPIIRCS RGTAAEMVAV KLDKKLRENL RDARNSLFTG DPLGTGQFSF QRPLLVLVDR NIDLATPLHH TWTYQALVHD VLDFHLNRVN LEESTGVENS PAGARPKRKN KKSYDLTPVD KFWQKHKGSP FPEVAESVQQ ELESYRAQED EVKRLKSIMG LEGEDEGAIS MLSDNTAKLT SAVSSLPELL EKKRLIDLHT NVATAVLEHI KARKLDVYFE YEEKIMSKTT LDKSLLDVIS DPDAGTPEDK MRLFLIYYIS AQQAPSEVDL EQYKKALTDA GCNLSPLQYI KQWKAFAKMA STPASYGNTT TKPMGLLSRV MNTGSQFVME GVKNLVLKQQ NLPVTRILDN LMEMKSNPET DDYRYFDPKM LRSNDSSVPR NKSPFQEAIV FVVGGGNYIE YQNLVDYIKA KQGKHILYGC SEIFNATQFI KQLSQLGQK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- SCFD1 (Sec1 Family Domain Containing 1 (SCFD1))
- Andere Bezeichnung
- Scfd1 (SCFD1 Produkte)
- Synonyme
- DDBDRAFT_0188070 Protein, DDBDRAFT_0234142 Protein, DDB_0188070 Protein, DDB_0234142 Protein, C14orf163 Protein, RA410 Protein, SLY1 Protein, SLY1P Protein, STXBP1L2 Protein, 3110021P21Rik Protein, Sly1 Protein, rSly1 Protein, sec1 family domain containing 1 Protein, vesicle transport-related protein Protein, Sec1-like family protein Protein, sec1 family domain-containing protein 1 Protein, sec1 family domain containing 1 L homeolog Protein, Sec1 family domain containing 1 Protein, SCFD1 Protein, PVX_111025 Protein, scfd1 Protein, LOC100639066 Protein, scfd1.L Protein, Scfd1 Protein
- Hintergrund
- Sec1 family domain-containing protein 1 (Syntaxin-binding protein 1-like 2),FUNCTION: Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity). {ECO:0000250}.
- Molekulargewicht
- 72.3 kDa
- UniProt
- Q8BRF7
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