AEN Protein (AA 1-336) (Strep Tag)
-
- Target Alle AEN Proteine anzeigen
- AEN (Apoptosis Enhancing Nuclease (AEN))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-336
-
Spezies
- Maus
-
Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses AEN Protein ist gelabelt mit Strep Tag.
- Applikation
- SDS-PAGE (SDS), ELISA, Western Blotting (WB)
- Sequenz
-
MVAGEVPEST QCPSLTSLNT KDVVRRRHKR RSRQHQRFMA RKALLQEQEL LSTAPGPGLC LLPSPSQMPA VTEASDSRRQ RPKARSGSNG LCSKKSVPRE APRPGPIKCV AIDCEMVGTG PQGRVSELAR CSVVSYSGDV LYDKYIRPEM PIVDYRTRWS GITRQHMHKA IPFQVAQKEI LKLLKGKVVV GHALHNDFQA LKYVHPRSQT RDTTYVPNLL SQPSSLIRTR VSLKDLALNL LHKKIQVGHQ GHSSVEDAMT AMELYQLVEV QWEQQVASSA QAPAEDRGPD SSTDVEQYMD DQYWPEDLTQ STGAETNGGP DRQEGEEGQG ARSAPP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
-
-
- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
-
- Target
- AEN (Apoptosis Enhancing Nuclease (AEN))
- Andere Bezeichnung
- Aen (AEN Produkte)
- Synonyme
- AEN Protein, ISG20L1 Protein, xaen Protein, isg20l1 Protein, MGC147218 Protein, pp12744 Protein, 2700083B06Rik Protein, Isg20l1 Protein, RGD1305051 Protein, apoptosis enhancing nuclease Protein, apoptosis enhancing nuclease L homeolog Protein, AEN Protein, aen.L Protein, aen Protein, Aen Protein
- Hintergrund
- Apoptosis-enhancing nuclease (EC 3.1.-.-) (Interferon-stimulated 20 kDa exonuclease-like 1),FUNCTION: Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis (By similarity). {ECO:0000250}.
- Molekulargewicht
- 37.3 kDa
- UniProt
- Q9CZI9
-