APBA1 Protein (AA 1-842) (Strep Tag)
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- Target Alle APBA1 Produkte
- APBA1 (Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 1 (APBA1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-842
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses APBA1 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
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MNHLEGSAEV EVADEAPGGE VNESVEADLE HPEVVEGQQP SPSPPPPAGH EPEDHRGHPA PPPPPPPQEE EEEERGECLA RSASTESGFH NHTDTAEGDV LAAARDGYEA ERAQDADDES AYAVQYRPEA EEYTEQAEAE HVEAAQRRAL PNHLHFHSLE HEEAMNAAYS GYVYTHRLFH RAEDEPYAEP YADYGGLQEH VYEEIGDAPE LEARDGLRLY ERERDEAAAY RQEALGARLH HYDERSDGES DSPEKEAEFA PYPRMDSYEQ EEDIDQIVAE VKQSMSSQSL DKAAEDMPEA EQDLERAPTP GGGHPDSPGL PAPAGQQQRV VGTPGGSEVG QRYSKEKRDA ISLAIKDIKE AIEEVKTRTI RSPYTPDEPK EPIWVMRQDI SPTRDCDDQR PVDGDSPSPG SSSPLGAESS SIPLHPGDPT EASTNKESRK SLASFPTYVE VPGPCDPEDL IDGIIFAANY LGSTQLLSDK TPSKNVRMMQ AQEAVSRIKT AQKLAKSRKK APEGESQPMT EVDLFISTQR IKVLNADTQE PMMDHPLRTI SYIADIGNIV VLMARRRMPR SNSQENVEAS HPSQDGKRQY KMICHVFESE DAQLIAQSIG QAFSVAYQEF LRANGINPED LSQKEYSDLL NTQDMYNDDL IHFSKSENCK DVFIEKQKGE ILGVVIVESG WGSILPTVII ANMMHGGPAE KSGKLNIGDQ IMSINGTSLV GLPLSTCQSI IKGLKNQSRV KLNIVRCPPV TTVLIRRPDL RYQLGFSVQN GIICSLMRGG IAERGGVRVG HRIIEINGQS VVATPHEKIV HILSNAVGEI HMKTMPAAMY RLLTAQEQPV YI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- APBA1 (Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 1 (APBA1))
- Andere Bezeichnung
- Apba1 (APBA1 Produkte)
- Synonyme
- APBA1 Protein, 6430513E09Rik Protein, Lin-10 Protein, Mint Protein, Mint1 Protein, Urop11 Protein, X11 Protein, X11alpha Protein, D9S411E Protein, LIN10 Protein, MINT1 Protein, X11A Protein, X11ALPHA Protein, amyloid beta precursor protein binding family A member 1 Protein, amyloid beta (A4) precursor protein-binding, family A, member 1b Protein, ketopantoate reductase Protein, 2-dehydropantoate 2-reductase Protein, amyloid beta (A4) precursor protein-binding, family A, member 1 (X11) Protein, amyloid beta (A4) precursor protein-binding, family A, member 1 Protein, amyloid beta (A4) precursor protein binding, family A, member 1 Protein, APBA1 Protein, apba1b Protein, apbA1 Protein, apba1 Protein, Apba1 Protein
- Hintergrund
- Amyloid-beta A4 precursor protein-binding family A member 1 (Adapter protein X11alpha) (Neuron-specific X11 protein) (Neuronal Munc18-1-interacting protein 1) (Mint-1),FUNCTION: Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor protein (APP) and hence formation of AAP-beta (By similarity). Component of the LIN-10-LIN-2-LIN-7 complex, which associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (PubMed:10846156). {ECO:0000250, ECO:0000269|PubMed:10846156}.
- Molekulargewicht
- 92.9 kDa
- UniProt
- B2RUJ5
- Pathways
- Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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