CBFA2T3 Protein (AA 1-620) (Strep Tag)
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- Target Alle CBFA2T3 Proteine anzeigen
- CBFA2T3 (Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 3 (CBFA2T3))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-620
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses CBFA2T3 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MSQASTTTLE SGALLSGPRG LQYGSPAHRK EKAAAMPDSP AEVKTQPRST PPSMPPPPPT SSQGATRPPS FTPHTHGEDG PATSLPHGRF HGCLKWSMVC LLMNGSSHSP TAIHGAPSTP NGFSNGPATS STASLSTQHL PPACGARQLS KLKRFLTTLQ QFGSDISPEI GERVRTLVLG LVNSTLTIEE FHAKLQEATN FPLRPFVIPF LKANLPLLQR ELLHCARLAK QTPAQYLAQH EQLLLDASAT SPVDSSELLL EVNENGKRRT PDRTKENGSD RDPLHPDHLS KRSCTLSPAQ RCSPSNGLPH PTPPPPPHYR LEDMAMAHHF RDSYRHPDPR ELRERHRPLA IPGSRQEEVI DHRLTEREWA EEWKHLNSLL NCIMDMVEKT RRSLTVLRRC QEADREELNH WIRCYSDSEE GKKGPTPISA RSLNSCSGPE GSQLDVHRDF TPRTLSGYMP EEIWRKAEEA VNEVKRQAMS ELQKAVSDAE RKAHELITTE RAKMERALAE AKRQASEDAL TVINQQEDSS ESCWNCGRKA SETCSGCNAA RYCGSFCQHK DWEKHHHVCG QSLQGPAAAV ADPLPGQPDA TASPSEAGSA GPSRPCSPGP PGPLDAAVPR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product CBFA2T3 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- CBFA2T3 (Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 3 (CBFA2T3))
- Andere Bezeichnung
- Cbfa2t3 (CBFA2T3 Produkte)
- Synonyme
- A630044F12Rik Protein, AI465270 Protein, AW229127 Protein, Cbfa2t3h Protein, ETO-2 Protein, Eto2 Protein, MTGR2 Protein, MTG16a Protein, MTG16b Protein, ETO2 Protein, MTG16 Protein, ZMYND4 Protein, core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Protein, CBFA2/RUNX1 translocation partner 3 Protein, Cbfa2t3 Protein, CBFA2T3 Protein
- Hintergrund
- Protein CBFA2T3 (Eight twenty one protein 2) (MTG8-related protein 2) (Protein ETO-2),FUNCTION: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A, interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (By similarity). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:16407974). Plays a role in granulocyte differentiation (PubMed:15231665). {ECO:0000250|UniProtKB:O75081, ECO:0000269|PubMed:11533236, ECO:0000269|PubMed:15231665, ECO:0000269|PubMed:16407974}.
- Molekulargewicht
- 68.0 kDa
- UniProt
- O54972
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