Dnmt2 Protein (AA 1-415) (Strep Tag)
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- Target Alle Dnmt2 (TRDMT1) Proteine anzeigen
- Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-415
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Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses Dnmt2 Protein ist gelabelt mit Strep Tag.
- Applikation
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Sequenz
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MEPLRVLELY SGIGGMHHAL RESHIPAHVV AAIDVNTVAN EVYKHNFPHT HLLSKTIEGI SLEDFDKLSF NMILMSPPCQ PFTRIGLQGD MTDPRTTSFL YILDILPRLQ KLPKYILLEN VKGFEVSSTR GLLIQTIEAC GFQYQEFLLS PSSLGIPNSR LRYFLIAKLQ SEPFPFQAPG QILMEFPKIV TVEPQKYAVV EESQPRVQRT GPRICAESSS TQSSGKDTIL FKLETVEERD RKHQQDSDLS VQMLKDFLED GDTDEYLLPP KLLLRYALLL DIVKPTSRRS MCFTKGYGSY IEGTGSVLQA AEDAQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN LQGFPPEFGF PEKTTVKQRY RLLGNSLNVH VVAKLLTVLC EGFGNASESC HKMPLILDSN SKILS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product TRDMT1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))
- Andere Bezeichnung
- Trdmt1 (TRDMT1 Produkte)
- Synonyme
- MGC89267 Protein, DMNT2 Protein, DNMT2 Protein, MHSAIIP Protein, PUMET Protein, RNMT1 Protein, Dnmt2 Protein, Rnmt2 Protein, dnmt2 Protein, tRNA aspartic acid methyltransferase 1 Protein, tRNA aspartic acid methyltransferase 1 L homeolog Protein, trdmt1 Protein, TRDMT1 Protein, Trdmt1 Protein, trdmt1.L Protein
- Hintergrund
- TRNA (cytosine(38)-C(5))-methyltransferase (EC 2.1.1.204) (DNA (cytosine-5)-methyltransferase-like protein 2) (Dnmt2) (DNA methyltransferase homolog MmuIIP) (DNA MTase homolog MmuIIP) (M.MmuIIP) (Met-2),FUNCTION: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) (PubMed:21183079, PubMed:22885326, PubMed:26271101). Has higher activity on tRNA(Asp) modified with queuosine at position 34 (By similarity). {ECO:0000250|UniProtKB:O14717, ECO:0000269|PubMed:21183079, ECO:0000269|PubMed:22885326, ECO:0000269|PubMed:26271101}.
- Molekulargewicht
- 46.8 kDa
- UniProt
- O55055
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