PAX3 and PAX7 Binding Protein 1 (PAXBP1) (AA 1-919) protein (Strep Tag)
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- Target Alle PAX3 and PAX7 Binding Protein 1 (PAXBP1) Proteine anzeigen
- PAX3 and PAX7 Binding Protein 1 (PAXBP1)
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-919
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Strep Tag
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MFRKARRVNV RKRNDSEEEE RERDEEQEPP PLLPPPASGE EPGPGGGDRA PAGESLLGPG PLPPPPSAHH PGLGAEAGGG ISGGAEPGNG LKPRKRPREN KEVPRASLLS FQDEEEENEE VFKVKKSSYS KKIVKLLKKE YKEDLEKSKI KTELNTAADS DQPLDKTCHA KDTNPEDGVV ISEHGEDEMD MESEKEEEKP KAGGAFSNAL SSLNVLRPGE IPDAAFIHAA RKKRQLAREL GDFTPHDSEP GKGRLVREDE NDASDDEDDD EKRRIVFSVK EKSQRQKIAE EIGIEGSDDD ALVTGEQDEE LSRWEQEQIR KGINIPQVQA SQPSEVNVYY QNTYQTMPYG ASYGIPYSYT AYGSSDAKSQ KTDNTVPFKT PSNEMAPVTI DLVKRQLKDR LDSMKELHKT NQQQHEKHLQ SRVDSTRAIE RLEGSSGGIG ERYKFLQEMR GYVQDLLECF SEKVPLINEL ESAIHQLYKQ RASRLVQRRQ DDIKDESSEF SSHSNKALMA PNLDSFGRDR ALYQEHAKRR IAEREARRTR RRQAREQTGQ MADHLEGLSS DDEETSTDIT NFNLEKDRIL KESSKVFEDV LESFYSIDCI KAQFEAWRSK YYMSYKDAYI GLCLPKLFNP LIRLQLLTWT PLEAKCRDFE TMLWFESLLF YGCEDREQEK DEADVALLPT IVEKVILPKL TVIAETMWDP FSTTQTSRMV GITMKLINGY PSVVNADNKN TQVYLKALLL RMRRTLDDDV FMPLYPKNVL ENKNSGPYLF FQRQFWSSVK LLGNFLQWYG IFSNKTLQEL SIDGLLNRYI LMAFQNSEYG DDSIRKAQNV INCFPKQWFV NLKGERTISQ LENFCRYLVH LADTIYRNSI GCSDVEKRNA RENIKQIVKL LASVRALDHA ISVASDHNVK EVKSLIEGK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product PAXBP1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- PAX3 and PAX7 Binding Protein 1 (PAXBP1)
- Andere Bezeichnung
- Paxbp1 (PAXBP1 Produkte)
- Synonyme
- BM020 Protein, C21orf66 Protein, FSAP105 Protein, GCFC Protein, GCFC1 Protein, 1810007M14Rik Protein, Gcfc Protein, Gcfc1 Protein, Pax3/7bp Protein, bm020 Protein, c21orf66 Protein, fsap105 Protein, gcfc Protein, gcfc1 Protein, PAX3 and PAX7 binding protein 1 Protein, PAX3 and PAX7 binding protein 1 L homeolog Protein, PAXBP1 Protein, Paxbp1 Protein, paxbp1.L Protein
- Hintergrund
- PAX3- and PAX7-binding protein 1 (PAX3/7BP) (GC-rich sequence DNA-binding factor 1),FUNCTION: Adapter protein linking the transcription factors PAX3 and PAX7 to the histone methylation machinery and involved in myogenesis. Associates with a histone methyltransferase complex that specifically mediates dimethylation and trimethylation of 'Lys-4' of histone H3. Mediates the recruitment of that complex to the transcription factors PAX3 and PAX7 on chromatin to regulate the expression of genes involved in muscle progenitor cells proliferation including ID3 and CDC20. {ECO:0000269|PubMed:22862948}.
- Molekulargewicht
- 104.8 kDa
- UniProt
- P58501
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