RAD54L Protein (AA 1-747) (Strep Tag)
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- Target Alle RAD54L Proteine anzeigen
- RAD54L (RAD54-Like (RAD54L))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-747
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses RAD54L Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MRRSLAPSQL ARRKPEDRSS DDEDWQPGTV TPKKRKSSSE TQVQECFLSP FRKPLTQLLN RPPCLDSSQH EAFIRSILSK PFKVPIPNYQ GPLGSRALGL KRAGVRRALH DPLEEGALVL YEPPPLSAHD QLKLDKEKLP VHVVVDPILS KVLRPHQREG VKFLWECVTS RRIPGSHGCI MADEMGLGKT LQCITLMWTL LRQSPECKPE IEKAVVVSPS SLVKNWYNEV EKWLGGRIQP LAIDGGSKDE IDRKLEGFMN QRGARVPSPI LIISYETFRL HVGVLKKGNV GLVICDEGHR LKNSENQTYQ ALDSLNTSRR VLISGTPIQN DLLEYFSLVH FVNSGILGTA HEFKKHFELP ILKSRDAAAS EADRQRGEER LRELIGIVNR CLIRRTSDIL SKYLPVKIEQ VVCCRLTPLQ TELYKRFLRQ AKPEEELREG KMSVSSLSSI TSLKKLCNHP ALIYDKCVAE EDGFEGTLGI FPPGYNSKAV EPQLSGKMLV LDYILAVTRS RSSDKVVLVS NYTQTLDLFE KLCRVRRYLY VRLDGTMSIK KRAKVVERFN SPSSPDFVFM LSSKAGGCGL NLIGANRLVM FDPDWNPAND EQAMARVWRD GQKKICYIYR LLSAGTIEEK IFQRQSHKKA LSSCVVDEEQ DVERHFSLGE LKELFTLDEA SLSDTHDRLH CRRCVNNRQV WPPPDGSDCT SDLAQWNHST DKRGLQDEVL QAAWDASSTA ITFVFHQRSH EEQRGLH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product RAD54L Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- RAD54L (RAD54-Like (RAD54L))
- Andere Bezeichnung
- Rad54l (RAD54L Produkte)
- Synonyme
- HR54 Protein, RAD54A Protein, hHR54 Protein, hRAD54 Protein, RAD54 Protein, zgc:56289 Protein, rad54l Protein, MGC69368 Protein, GdRAD54 Protein, RAD54-like Protein, RAD54L Protein, RAD54 like Protein, RAD54 like (S. cerevisiae) Protein, RAD54-like (S. cerevisiae) Protein, RAD54L Protein, Rad54l Protein, rad54l Protein
- Hintergrund
- DNA repair and recombination protein RAD54-like (EC 3.6.4.12) (RAD54 homolog) (mHR54) (mRAD54),FUNCTION: Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Plays also an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (By similarity). Deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role in telomere capping. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. LIG4 and RAD54L cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations (PubMed:10209103, PubMed:10757799, PubMed:12218123, PubMed:12531026, PubMed:12548566, PubMed:12897131, PubMed:15175260, PubMed:9108475). {ECO:0000250|UniProtKB:P32863, ECO:0000269|PubMed:10209103, ECO:0000269|PubMed:10757799, ECO:0000269|PubMed:12218123, ECO:0000269|PubMed:12531026, ECO:0000269|PubMed:12548566, ECO:0000269|PubMed:12897131, ECO:0000269|PubMed:15175260, ECO:0000269|PubMed:9108475}.
- Molekulargewicht
- 84.7 kDa
- UniProt
- P70270
- Pathways
- DNA Reparatur
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