ARHGAP20 Protein (AA 1-1182) (Strep Tag)
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- Target Alle ARHGAP20 Produkte
- ARHGAP20 (rho GTPase Activating Protein 20 (ARHGAP20))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-1182
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses ARHGAP20 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
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MEAMSPQQDA LGAQPGRSSS LTGMSRIAGG PGTKKKMKTL AERRRSAPSL ILDKALQKRP STRDSHSASI DTCAFLSSFM CSSRTLLIDG PVELKRGLQR QERHLFLFND LFVSAKIKYN NNFKIKNKIR LTDMWTASCV EEVGEGNMNA QKSFVLGWPT VNFVATFSSP EQKDKWLSLL QRYIALEKEK DYPKSIPLKI FAKDIGNCAY FKTITVMNSD TASEVINMSL QMLGITGSER DYQLWVNSGK EAAPYPLIGH EYPYGIKMSH LRDTALLTQG SRDSASPSQL QEPFLMEQLP REMQCQFILK PTRLATAQQL SDSSQKTFKR RRSIINWAFW RGSSTHLDNL PMSPTSPMPG QLFGVSLPDL CENDNLPKPI LDMLSFLNQK GPLTKGIFRQ SANMKSCREL KEKLNSGIEV HLDCESIFVI ASVLKDFLRN IPESIFSSDL YDHWVCVMDQ GNDEEKINII QRLLDQLPRA NVVFLRYLFG VLHNIEQHSL SNQMTAFNLA VCIAPSILWP PASSSPELEN EFTKKVSLLI QFLIENCCRV FGEEIASLLG ELSERSDREH TPDITCFQMN DSSYDSLENE LNEEADAPCS DLVKKLGQGS RSMDSVLTLS DYDLEQPEVE GLLTLSNFDL DQSKEEHIPI KPPLEPKPVN VFVGYRKVSL GEHARAPAGP GTLSCLPVAA ADAPKVLRRH RRSSEPSIDY LDTKLSYLRE FYQKKLRKSS CDAVLSRKDE DYLKQTQPQK KGDKVCLKQS SVTGTDVSKR NTANENIKKK SLSGHEGTQV TLFTKSKPVP ISVASYSHGS SQDHPRKQAF DADPCRFSPP HLTDAQKSSR VQHRRCSEPS IDDQNYKLSY LRGIYSMKQN KASCEAGLLH GEDDYLRRHK SLQIEGQKLI NQSLVMGIEV GKSSSSHQST EKVLPPRLNL CPRASYSSLS SPGSSPSGSS VSSQDSAFSQ ISEHSVFTPT ETSSPIDCTF QTQRKQEELS SDFDSPSRLS GMPGPSMGQA SSHLAYLRKG TTEQPSQMHS VTLHPSAWLR SGLVTLKNWS LKKKTKAARP EDRKVCSLKE PLELPSCASG TPEADSLQES QDDLQGDEGP GQTACGFSSY ACQDSEQHAG SPFHLAESRL KPCMKLYKGE ESGGQYPCDN PWEGASSSLE TTEDTANPGA EPTTFAMTGT DI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ARHGAP20 (rho GTPase Activating Protein 20 (ARHGAP20))
- Andere Bezeichnung
- Arhgap20 (ARHGAP20 Produkte)
- Synonyme
- ARHGAP20 Protein, rarhogap Protein, MGC145445 Protein, fk54b10 Protein, wu:fk54b10 Protein, RARHOGAP Protein, RARhoGAP Protein, 6530403F17Rik Protein, A530023E23Rik Protein, mKIAA1391 Protein, Rho GTPase activating protein 20 Protein, ARHGAP20 Protein, arhgap20 Protein, Arhgap20 Protein
- Hintergrund
- Rho GTPase-activating protein 20 (RA and RhoGAP domain-containing protein) (RARhoGAP) (Rho-type GTPase-activating protein 20),FUNCTION: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. {ECO:0000250}.
- Molekulargewicht
- 131.4 kDa
- UniProt
- Q6IFT4
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