RFX6 Protein (AA 1-927) (Strep Tag)
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- Target Alle RFX6 Proteine anzeigen
- RFX6 (Regulatory Factor X 6 (RFX6))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-927
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses RFX6 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MAKVRELEEA FVQEQPSPQL PSEIAEECCA QLLGKGLLVY PEDSAYLLAE TAAGARSSGE KGGDPGLQVG VKSEMQLNNG NFSSEEEDAD TQESKTKAAD PQLSQKKSIT QMMKDKKKQT QLTLQWLEDN YIVCEGVCLP RCILYAHYLD FCRKEKLEPA CAATFGKTIR QKFPLLTTRR LGTRGHSKYH YYGIGIKESS AYYHSVYSGK GLTRFSGSKL KNEGGFTRKY SLSSKTGTLL PEFPSAQHLV YQGCISKDKV DTLIMMYKTH CQCILDNAIN GNFEEIQHFL LHFWQGMPDH LLPLLENPVI IDIFCVCDSI LYKVLTDVLI PATMQEMPES LLADIRNFAK NWEQWVVSSL ENLPEALIDK KIPILRRFVS SLKRQTSFLH LAQIARPALF DQHVVNAMVS DIEKVDLNSI GSQALLTISN STDTESDIYS EHDSITVFQE LKDLLKKNAT VEAFIEWLDT VVEQRVIKMS KQNGRSLKKR AQDFLLKWSF FGARVMHNLT LNNASSFGSF HLIRMLLDEY ILLAMETQFN NDKEQELQNL LDKYMKNSDA SKAAFTASPS SCFLANRNKA SSLASDTVKN ESHVETSYVP LPSSQPGAIP PALHPFSTED TDNMPLPGQI ELSQSTGHLM TPPISPAIAS RGSVINQGPM ASRPPSVGTV LSAPTHCSTY AEPIYPTLSP ANHDFYGTNS NYQTMFRTQS HPASSLYAHR AEHGRCMAWT EQQLSRDFFG GSCAGSPYNC RPPSSYGPST HTQESHSMQV LNTGSFNFLS NAGAGSCQGS TLPSNSPNGY YGNNINYSEA HRLGSMVNQH VSVISSVRSL PPYSDIHDPL NILDDSSRKQ NNSFYADTLS PVACRTTVVA SNLQTQIPSS SSQCMYGTSN QYPVQDSLDS NAASNREMVS SLPPINTVFM GTAAGDT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product RFX6 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- RFX6 (Regulatory Factor X 6 (RFX6))
- Andere Bezeichnung
- Rfx6 (RFX6 Produkte)
- Synonyme
- MTFS Protein, RFXDC1 Protein, dJ955L16.1 Protein, 4930572O07Rik Protein, Rfxdc1 Protein, regulatory factor X6 Protein, regulatory factor X, 6 Protein, RFX6 Protein, Rfx6 Protein
- Hintergrund
- DNA-binding protein RFX6 (Regulatory factor X 6) (Regulatory factor X domain-containing protein 1),FUNCTION: Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed:20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (By similarity). {ECO:0000250|UniProtKB:Q8HWS3, ECO:0000269|PubMed:20148032}.
- Molekulargewicht
- 102.6 kDa
- UniProt
- Q8C7R7
- Pathways
- Carbohydrate Homeostasis
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