EXOC6 Protein (AA 1-802) (Strep Tag)
-
- Target Alle EXOC6 Proteine anzeigen
- EXOC6 (Exocyst Complex Component 6 (EXOC6))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-802
- Spezies
- Maus
-
Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses EXOC6 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
-
MAESCEALGT VPEHERILQE IESTDTACVG PTLRSVYDDQ PNAHKKFMEK LDACIRNHDK EIEKMCNFHH QGFVDAITEL LKVRADAEKL KVTDTNRRFQ DAGKEVIIQT EDIIRCRIQQ RNITTVVEKL QLCLPVLEMY SKLKEQMSMK RYYSALKTME QLENVYFPRV SQYRFCQLMM ETLPKLREDM MNYCMSDLTY GLESIRKHSD KIGEAAMKQA QQQKSFSVAL QKQNNMRFGK NMHVNNDRIL EEKSDVIPKH ALEEEAENDE EVLTVQDLVD FSPVYRCLHI YSALGDEETF ENYYRKQRKK QARLVLQPQS SVHETVDGYR RYFTQIVGFF VVEDHILHVT QGLVTRVYTE ELWNMALSKI IAVLRAHSSY CTDPDLVLEL KNLIVIFADT LQGYGFPVNR LFDLLFEIRD QYNETLLKKW AGIFRDIFEE DNYSPIPIGS EEEYKVVISR FPFQDPDLEK QSFPKKFPMS QSVPLIYIQV KEFIYASLKF SESLHRSSTE IDDMLRKSTN LLLTRILSSC LLNLIRKPHI GLTELVQIII NTTHLEQACK YLEDFITNIT NISQETVHTT RLYGLSTFKD ARHAAEGEIY TKLNQKIDEF VQLADYDWTM AESDGRASGY LMDLINFLRS IFQVFTHLPG KVAQTACMSA CQHLSTSLMQ MLLDSELKQI SMGAVQQFNL DVIQCELFAS SEPVPGFQGD TLQLAFIDLR QLLDLFMVWD WSTYLADYGQ PASKYLRVNP HAALTLLEKM KDTSKKNNIF AQFRKNDRDR QKLIETVVRQ LRGLVTGMSQ HT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
-
-
- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
-
- Target
- EXOC6 (Exocyst Complex Component 6 (EXOC6))
- Andere Bezeichnung
- Exoc6 (EXOC6 Produkte)
- Synonyme
- Sec15 Protein, Sec15l1 Protein, EXOC6A Protein, SEC15 Protein, SEC15L Protein, SEC15L1 Protein, SEC15L3 Protein, Sec15p Protein, 4833405E05Rik Protein, AW413330 Protein, C430002C19 Protein, hbd Protein, msec15 Protein, zgc:77548 Protein, SEC15A Protein, EXOC6 Protein, 3R41 Protein, CG7034 Protein, Dmel\\CG7034 Protein, dsec15 Protein, exocyst complex component 6 Protein, exocyst complex component 6B Protein, Exocyst complex component 6 Protein, Secretory 15 Protein, Exoc6 Protein, EXOC6 Protein, exoc6 Protein, LOC410084 Protein, EXOC6B Protein, CpipJ_CPIJ005438 Protein, Tsp_03288 Protein, sec-15 Protein, Sec15 Protein
- Hintergrund
- Exocyst complex component 6 (Exocyst complex component Sec15A) (SEC15-like protein 1),FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Together with RAB11A, RAB3IP, RAB8A, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. {ECO:0000250}.
- Molekulargewicht
- 93.1 kDa
- UniProt
- Q8R313
- Pathways
- Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis
-