GCNT1 Protein (AA 1-428) (Strep Tag)
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- Target Alle GCNT1 Proteine anzeigen
- GCNT1 (Glucosaminyl (N-Acetyl) Transferase 1, Core 2 (GCNT1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-428
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses GCNT1 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MLRNLFRRRL FSCPTKYYFM LLVLSLITFS VLRIHQKPEF FSVRHLELAG DDPYSNVNCT KILQGDPEEI QKVKLEILTV QFKKRPRRTP HDYINMTRDC ASFIRTRKYI VEPLTKEEVG FPIAYSIVVH HKIEMLDRLL RAIYMPQNFY CIHVDRKAEE SFLAAVQGIA SCFDNVFVAS QLESVVYASW SRVKADLNCM KDLYRMNANW KYLINLCGMD FPIKTNLEIV RKLKCSTGEN NLETEKMPPN KEERWKKRYT VVDGKLTNTG IVKAPPPLKT PLFSGSAYFV VTREYVGYVL ENENIQKLME WAQDTYSPDE FLWATIQRIP EVPGSFPSSN KYDLSDMNAI ARFVKWQYFE GHVSNGAPYP PCSGVHVRSV CVFGAGDLSW MLRQHHLFAN KFDMDVDPFA IQCLDEHLRH KALENLEH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product GCNT1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- GCNT1 (Glucosaminyl (N-Acetyl) Transferase 1, Core 2 (GCNT1))
- Andere Bezeichnung
- Gcnt1 (GCNT1 Produkte)
- Synonyme
- C2GNT Protein, C2GNT-L Protein, C2GNT1 Protein, G6NT Protein, NACGT2 Protein, NAGCT2 Protein, 5630400D21Rik Protein, B130048E03 Protein, C2 GlcNAcT Protein, IGnT Protein, core 2 GlcNAc-T Protein, glucosaminyl (N-acetyl) transferase 1, core 2 L homeolog Protein, glucosaminyl (N-acetyl) transferase 1, core 2 Protein, gcnt1.L Protein, GCNT1 Protein, Gcnt1 Protein
- Hintergrund
- Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.102) (Core 2 beta-1,6-N-acetylglucosaminyltransferase) (C2GlcNAcT) (Core 2-branching enzyme) (Core2-GlcNAc-transferase) (C2GNT) (Leukocyte type core 2 beta-1,6-N-acetylglucosaminyltransferase) (C2GnT-L),FUNCTION: Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. The catalysis is metal ion-independent and occurs with inversion of the anomeric configuration of sugar donor (PubMed:9881978, PubMed:12954635, PubMed:22056345). Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites (PubMed:9881978). Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage-specific embryonic antigen 1 (SSEA-1) determinant (PubMed:7983056). Can use Galbeta1-3GalNAcalpha1-R and Galbeta1-3GalNAcbeta1-R oligosaccharide derivatives as acceptor substrates (PubMed:7983056). {ECO:0000269|PubMed:12954635, ECO:0000269|PubMed:22056345, ECO:0000269|PubMed:7983056, ECO:0000269|PubMed:9881978}.
- Molekulargewicht
- 49.8 kDa
- UniProt
- Q09324
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