GPR84 Protein (AA 1-396) (Strep Tag)
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- Target Alle GPR84 Proteine anzeigen
- GPR84 (G Protein-Coupled Receptor 84 (GPR84))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-396
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses GPR84 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequenz
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MWNSSDANFS CYHESVLGYR YFAVIWGVAV AVTGTVGNVL TLLALAIRPK LRTRFNLLIA NLTLADLLYC TLLQPFSVDT YLHLHWRTGA VFCRIFGLLL FTSNSVSILT LCLIALGRYL LIAHPKLFPQ VFSAKGIVLA LVGSWVVGVT SFAPLWNVFV LVPVVCTCSF DRMRGRPYTT ILMGIYFVLG LSSVGVFYCL IHRQVKRAAR ALDQYGLHQA SIRSHQVAGT QEAMPGHFQE LDSGVASRGP SEGISSEPVS AATTQTLEGD SSEAGGQGIR KAAQQIAERS LPEVHRKPRE TAGARRATDA PSEFGKVTRM CFAVFLCFAL SYIPFLLLNI LDARGRAPRV VHMVAANLTW LNSCINPVLY AAMNRQFRHA YGSILKRGPQ SFRRFH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product GPR84 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- GPR84 (G Protein-Coupled Receptor 84 (GPR84))
- Andere Bezeichnung
- Gpr84 (GPR84 Produkte)
- Synonyme
- EX33 Protein, GPCR4 Protein, GPR84 Protein, sb:eu355 Protein, zgc:171802 Protein, G protein-coupled receptor 84 Protein, G protein-coupled receptor 84 S homeolog Protein, GPR84 Protein, Gpr84 Protein, gpr84 Protein, gpr84.S Protein
- Hintergrund
- G-protein coupled receptor 84,FUNCTION: G protein-coupled receptor that responds endogenously to dietary fatty acids or nutrient, specifically medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists (By similarity). In immune cells, functions as a pro-inflammatory receptor via 6-OAU and promotes the expression of pro-inflammatory mediators such as TNFalpha, IL-6 and IL-12B as well as stimulating chemotactic responses through activation of signaling mediators AKT, ERK and NF-kappa-B (by sim). In addition, triggers increased bacterial adhesion and phagocytosis in macrophages and regulates pro-inflammatory function via enhancing NLRP3 inflammasome activation (PubMed:34912006). Plays also an important role in inflammation by modulating neutrophil functions (PubMed:37016043). Mechanistically, promotes neutrophil chemotaxis, reactive oxygen species (ROS) production and degranulation via LYN-AKT/ERK pathway (PubMed:37016043). To regulate ROS production, communicates with the two formyl peptide receptors FPR2 and FPR1 to control the NADPH oxidase activity in neutrophils (PubMed:29973940). {ECO:0000250|UniProtKB:Q9NQS5, ECO:0000269|PubMed:16966319}.
- Molekulargewicht
- 43.7 kDa
- UniProt
- Q8CIM5
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