CH25H Protein (AA 1-298) (Strep Tag)
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- Target Alle CH25H Proteine anzeigen
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-298
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses CH25H Protein ist gelabelt mit Strep Tag.
- Applikation
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Sequenz
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MGCYNGSELQ DLGCSSQLLL QPLWDTIRTR EAFTRSPIFP VTFSIITYVG FCLPFVVLDV LYPWVPILRR YKIHPDFSPS VKQLLPCLGL TLYQHLVFVF PVTLLHWVRS PALLPQEAPE LVQLLSHVLI CLLLFDTEIF AWHLLHHKVP WLYRTFHKVH HQNSSSFALA TQYMSFWELL SLTFFDVLNV AVLRCHPLTI FTFHVINIWL SVEDHSGYDF PWSTHRLVPF GWYGGVAHHD MHHSQFNCNF APYFTHWDKM LGTLRSAPLP ESLCACGERC VNSRERCAVH LIQKKKQT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product CH25H Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- Andere Bezeichnung
- Ch25h (CH25H Produkte)
- Synonyme
- CH25H Protein, zgc:101688 Protein, MGC143045 Protein, C25H Protein, AI462618 Protein, m25OH Protein, cholesterol 25-hydroxylase Protein, cholesterol 25-hydroxylase L homeolog Protein, CH25H Protein, ch25h Protein, Ch25h Protein, ch25h.L Protein
- Hintergrund
- Cholesterol 25-hydroxylase (EC 1.14.99.38) (Cholesterol 25-monooxygenase) (m25OH),FUNCTION: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097, PubMed:29033131). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed:9852097). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation (PubMed:9852097). Required to restrain inflammation in macrophages: production of 25-hydroxycholesterol protects macrophages from cholesterol overload, thereby preventing mitochondrial DNA release and subsequent activation of the AIM2 inflammasome (PubMed:29033131). Interferon-stimulated gene which has broad antiviral activities against a wide range of enveloped viruses (By similarity). {ECO:0000250|UniProtKB:O95992, ECO:0000269|PubMed:22999953, ECO:0000269|PubMed:29033131, ECO:0000269|PubMed:9852097}.
- Molekulargewicht
- 34.7 kDa
- UniProt
- Q9Z0F5
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