CNGA1 Protein (AA 1-684) (Strep Tag)
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- Target Alle CNGA1 Proteine anzeigen
- CNGA1 (Cyclic Nucleotide Gated Channel alpha 1 (CNGA1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-684
- Spezies
- Maus
- Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses CNGA1 Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
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MKTNIINTWH SFVNIPNVIV PAIEKEIRRM ENGACSSFSD DDNGSLSEES ENEDSFFRSN SYKRRGPSQR EQHLPGTMAL FNVNNSSNKD QEPKEKKKKK KEKKSKADDK NENKKDPEKK KKKEKEKEKK KKEEKTKEKK EEEKKEVVVI DPSGNTYYNW LFCITLPVMY NWTMIIARAC FDELQSDYLE YWLIFDYVSD VVYLADMFVR TRTGYLEQGL LVKDRMKLIE KYKANLQFKL DVLSVIPTDL LYIKFGWNYP EIRLNRLLRI SRMFEFFQRT ETRTNYPNIF RISNLVMYIV IIIHWNACVY YSISKAIGFG NDTWVYPDVN DPEFGRLARK YVYSLYWSTL TLTTIGETPP PVLDSEYIFV VVDFLIGVLI FATIVGNIGS MISNMNAARA EFQSRVDAIK QYMNFRNVSK DMEKRVIKWF DYLWTNKKTV DEREVLRYLP DKLRAEIAIN VHLDTLKKVR IFADCEAGLL VELVLKLQPQ VYSPGDYICK KGDIGREMYI IKEGKLAVVA DDGITQFVVL SDGSYFGEIS ILNIKGSKAG NRRTANIKSI GYSDLFCLSK DDLMEALTEY PDAKTMLEEK GRQILMKDGL LDINIANMGS DPKDLEEKVT RMEGSVDLLQ TRFARILAEY ESMQQKLKQR LTKVEKFLKP LIETEFSALE EPGGESELTE SLQD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- CNGA1 (Cyclic Nucleotide Gated Channel alpha 1 (CNGA1))
- Andere Bezeichnung
- Cnga1 (CNGA1 Produkte)
- Synonyme
- CNCG Protein, CNCG1 Protein, CNG-1 Protein, CNG1 Protein, RCNC1 Protein, RCNCa Protein, RCNCalpha Protein, RP49 Protein, CNG Protein, CNGC1 Protein, xcnga1 Protein, CNGA1 Protein, Cncg Protein, HCN Protein, cyclic nucleotide gated channel alpha 1 Protein, cyclic nucleotide gated channel alpha 1 L homeolog Protein, CNGA1 Protein, cnga1.L Protein, Cnga1 Protein
- Hintergrund
- CGMP-gated cation channel alpha-1 (Cyclic nucleotide-gated cation channel 1) (Cyclic nucleotide-gated channel alpha-1) (CNG channel alpha-1) (CNG-1) (CNG1) (Cyclic nucleotide-gated channel, photoreceptor) (Rod photoreceptor cGMP-gated channel subunit alpha),FUNCTION: Subunit of the rod cyclic GMP-gated cation channel, which is involved in the final stage of the phototransduction pathway. When light hits rod photoreceptors, cGMP concentrations decrease causing rapid closure of CNGA1/CNGB1 channels and, therefore, hyperpolarization of the membrane potential. {ECO:0000250|UniProtKB:Q00194}.
- Molekulargewicht
- 79.5 kDa
- UniProt
- P29974
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction, Asymmetric Protein Localization
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