ATP11A Protein (AA 1-1187) (Strep Tag)
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- Target Alle ATP11A Proteine anzeigen
- ATP11A (ATPase, Class VI, Type 11A (ATP11A))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-1187
- Spezies
- Maus
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Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses ATP11A Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
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MDCSLLRTLV RRYCAGEENW VDSRTIYVGH KEPPPGAEAY IPQRYPDNRI VSSKYTFWNF IPKNLFEQFR RIANFYFLII FLVQLIIDTP TSPVTSGLPL FFVITVTAIK QGYEDWLRHK ADNAMNQCPV HFIQHGKLVR KQSRKLRVGD IVMVKEDETF PCDLIFLSSN RADGTCHVTT ASLDGESSHK THYAVQDTKG FHTEADVDSL HATIECEQPQ PDLYKFVGRI NVYNDLNDPV VRPLGSENLL LRGATLKNTE KIFGVAIYTG METKMALNYQ SKSQKRSAVE KSMNTFLIVY LCILVSKALI NTVLKYVWQS EPFRDEPWYN EKTESERQRN LFLRAFTDFL AFMVLFNYII PVSMYVTVEM QKFLGSYFIT WDEDMFDEEM GEGPLVNTSD LNEELGQVEY IFTDKTGTLT ENNMAFKECC IEGHVYVPHV ICNGQVLPDS SGIDMIDSSP GVCGREREEL FFRAICLCHT VQVKDDHCGD DVDGPQKSPD AKSCVYISSS PDEVALVEGV QRLGFTYLRL KDNYMEILNR ENDIERFELL EVLTFDSVRR RMSVIVKSTT GEIYLFCKGA DSSIFPRVIE GKVDQVRSRV ERNAVEGLRT LCVAYKRLEP EQYEDACRLL QSAKVALQDR EKKLAEAYEQ IEKDLVLLGA TAVEDRLQEK AADTIEALQK AGIKVWVLTG DKMETASATC YACKLFRRST QLLELTTKKL EEQSLHDVLF DLSKTVLRCS GSMTRDSFSG LSTDMHDYGL IIDGAALSLI MKPREDGSSS GNYRELFLEI CRNCSAVLCC RMAPLQKAQI VKLIKFSKEH PITLAIGDGA NDVSMILEAH VGIGVIGKEG RQAARNSDYA IPKFKHLKKM LLVHGHFYYI RISELVQYFF YKNVCFIFPQ FLYQFFCGFS QQTLYDTAYL TLYNISFTSL PILLYSLMEQ HVGIDVLKRD PTLYRDIAKN ALLRWRVFIY WTFLGVFDAL VFFFGAYFIF ENTTVTINGQ MFGNWTFGTL VFTVMVLTVT LKLALDTHYW TWINHFVIWG SLLFYIAFSL LWGGVIWPFL SYQRMYYVFI SMLSSGPAWL GIILLVTVGL LPDVLKKVLC RQLWPTATER TQNIQHQDSI SEFTPLASLP SWGAQGSRLL AAQCSSPSGR VVCSRWESEE CPVLPLHPGL PHKARYGCCR SSLEMPT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin-Niveau
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ATP11A (ATPase, Class VI, Type 11A (ATP11A))
- Andere Bezeichnung
- Atp11a (ATP11A Produkte)
- Synonyme
- ATPIH Protein, ATPIS Protein, 4930558F19Rik Protein, AU040868 Protein, Atpc1h Protein, Ih Protein, Ua20 Protein, si:dkey-251i8.1 Protein, ATPase phospholipid transporting 11A Protein, ATPase, class VI, type 11A Protein, ATP11A Protein, Atp11a Protein, atp11a Protein
- Hintergrund
- Phospholipid-transporting ATPase IH (EC 7.6.2.1) (ATPase IS) (ATPase class VI type 11A) (P4-ATPase flippase complex alpha subunit ATP11A),FUNCTION: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of the plasma membrane (By similarity). Does not show flippase activity toward phosphatidylcholine (PC) (By similarity). Contributes to the maintenance of membrane lipid asymmetry with a specific role in morphogenesis of muscle cells. In myoblasts, mediates PS enrichment at the inner leaflet of plasma membrane, triggering PIEZO1-dependent Ca2+ influx and Rho GTPases signal transduction, subsequently leading to the assembly of cortical actomyosin fibers and myotube formation (PubMed:29799007). {ECO:0000250|UniProtKB:P98196, ECO:0000269|PubMed:29799007}.
- Molekulargewicht
- 135.5 kDa
- UniProt
- P98197
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