Recombinant Human Mannoside Acetylglucosaminyltransferase 2/MGAT2 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Arg30-Gln447) of Human MGAT2 fused with a 6His tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
MGAT2
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, ELISA, WB
MGAT2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl, pH 8.0 .
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
-80 °C
Informationen zur Lagerung
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Mannoside Acetylglucosaminyltransferase 2 (MGAT2) is a single-pass type II membrane protein that contains the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain and a C-terminal catalytic domain. MGAT2 catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. Defects in MGAT2 are the cause of congenital disorder of glycosylation type 2A. Alternative Names: Alpha-1,6-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase, Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, NT-II, Mannoside Acetylglucosaminyltransferase 2, N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase II,