Fluorescein isothiocyanate-conjugated bovine serum albumin, Cohn fraction V. Source: Pooled normal bovine serum
Aufreinigung
Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 20 mg/ml at the level of sensitivity of the applied immuno-precipitating techniques, this product contains traces transferrin and some alpha-proteins.
ALB
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
As an efficient counterstain for cell an tissue substrates with immunoconjugates with a label contrasting with the greenish-yellow fluorescence of BSA/FITC. The purpose of counterstaining is to enhance the optical contrast of the immuno-specific staining against a counterstained background.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Konzentration
BSA concentration 10 mg/ml. Fluorochrome/BSA molar ratio (F/P) 1.2.
Buffer
Fluorochrome-coupled bovine serum albumin, fraction V, lyophilized from a solution in phosphate buffered saline (PBS pH 7.2).
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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Hintergrund
Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to bovine total serum proteins, to bovine IgA, IgM and albumin