PPP1R1A
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
ELISA, SDS, WB, MS
ELISA, SDS-PAGE (SDS), Western Blotting (WB), Mass Spectrometry (MS)
Verwendungszweck
Recombinant Human PPP1R1A Protein is produced by our E. coli expression system and the target gene encoding Met1-Val171 is expressed with a 6His tag at the C-terminus.
PPP1R1A
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 50 % Glycerol, pH 8.5.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.