nectin-3 Protein (AA 58-400) (Fc Tag,AVI tag,Biotin)
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- Target Alle nectin-3 (NECTIN3) Proteine anzeigen
- nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 58-400
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses nectin-3 Protein ist gelabelt mit Fc Tag,AVI tag,Biotin.
- Marke
- PrecisionAvi
- Sequenz
- AA 58-400
- Spezifität
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Produktmerkmale
- This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 66.1 kDa. The protein migrates as 90-95 kDa and 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Reinheit
- >95 % as determined by SDS-PAGE.
- Endotoxin-Niveau
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product NECTIN3 Protein
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- Kommentare
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- Tris with Glycine, Arginine and NaCl, pH 7.5
- Handhabung
- Please avoid repeated freeze-thaw cycles.
- Lagerung
- -20 °C
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- Target
- nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
- Andere Bezeichnung
- Nectin-3 (NECTIN3 Produkte)
- Synonyme
- PVRL3 Protein, CD113 Protein, CDW113 Protein, NECTIN-3 Protein, PPR3 Protein, PRR3 Protein, PVRR3 Protein, 2610301B19Rik Protein, 3000002N23Rik Protein, 4921513D19Rik Protein, AA407785 Protein, AU016832 Protein, AW538082 Protein, nectin cell adhesion molecule 3 Protein, poliovirus receptor-related 3 Protein, Nectin3 Protein, NECTIN3 Protein, PVRL3 Protein, nectin3 Protein
- Hintergrund
- Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6, MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
- Molekulargewicht
- 66.1 kDa
- NCBI Accession
- NP_056295
- Pathways
- Cell-Cell Junction Organization
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