IDO1 Protein (AA 1-403) (His tag)
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- Target Alle IDO1 Proteine anzeigen
- IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-403
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses IDO1 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Kreuzreaktivität
- Human
- Produktmerkmale
- Human IDO (aa 1-403) is fused at the N-terminus to a His-tag.
- Reinheit
- >90 % (SDS-PAGE)
- Sterilität
- 0.2 μm filtered
- Endotoxin-Niveau
- <0.1EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product IDO1 Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Specific Activity: >100'000U/mg protein with L-tryptophan as substrate (activity assay with catalase). One unit is defined as the amount of enzyme that produces 1nmol of N-formylkynurenine (NFK) per hour. For more information see Activity Test Protocol.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- 0.2μm-filtered solution in 10 mM Tris, pH 7.5, containing 100 mM NaCl and 20 % glycerol.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
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Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 1 year after receipt when stored at -80°C. - Haltbarkeit
- 12 months
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- Target
- IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
- Andere Bezeichnung
- IDO (IDO1 Produkte)
- Synonyme
- INDO Protein, ido Protein, indo Protein, IDO Protein, cd107b Protein, IDO1 Protein, IDO-1 Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, indoleamine 2,3-dioxygenase 1 L homeolog Protein, indoleamine 2,3-dioxygenase 1-like Protein, IDO1 Protein, ido1.L Protein, ido1 Protein, Ido1 Protein, LOC100050681 Protein
- Hintergrund
- IDO is a heme enzyme that catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway, causing depletion of tryptophan which can lead to halted growth of microbes as well as T cells. IDO is an immune checkpoint protein, thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation and antioxidant activity. Cancer cells are able to evade the immune system is by hijacking the checkpoint proteins. Increased IDO protein levels drive growth arrest and apoptosis of the effector T cells, a group of immune cells that mediate the immune system's ability to destroy pathogens. By reducing the number of effector T cells, IDO overexpression prevents the immune system from effectively destroying cancer cells. IDO overexpression has been observed in a wide range of human cancers such as prostatic, colorectal, pancreatic, cervical, gastric, ovarian, head or lung cancer. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. Biological active IDO enzyme can be used for screening of IDO inhibitors, which have shown promising antitumor activity and appear to be synergistic in combination with chemotherapy and other forms of immunotherapy.
- Molekulargewicht
- ~47kDa (SDS-PAGE)
- UniProt
- P14902
- Pathways
- Activated T Cell Proliferation
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