Collagen IV Protein

Produktnummer ABIN5624596

Hersteller Produkt- Nr.: 009-001-106

Produktdetails

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Protein-Typ: Native

Spezies: Human

Quelle: Human

Applikation: ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-106

Hersteller: Rockland

Verwendungszweck: Human Collagen Type IV

Produktmerkmale: Type IV collagen, collagen 4, human collagen, Collagen alpha-4 (IV) chain

Aufreinigung: This product has been prepared from human placenta and is chromatographically and immunologically pure.  This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with anti-Collagen Type IV.  Reaction with Rockland's anti-Collagen I, II, III, V or VI is negligible.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Collagen type IV has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.5 M Acetic Acid

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 6 months

Antigendetails

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: COLLAGEN IV (COL4 Produkte)

Hintergrund: Background: Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.

UniProt: P02462