MEK1 Protein (pSer218, pSer222)

Produktnummer ABIN5624611

Produktdetails

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Protein-Typ: Recombinant

Proteineigenschaft: pSer218, pSer222

Spezies: Human, Maus, Ratte

Quelle: Insect cells (Sf9)

Applikation: Western Blotting (WB)

Aufreinigung: MEK1 pS218-pS222 is a recombinant protein containing a polyhistidine tag expressed in Sf9. This protein is expressed in the presense of constitutively active B-raf (V600E) leading to high levels of phosphorylation of MEK1 activation residues at position S218 and S222. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 95% pure.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise:

Application Note: MEK1 pS218-pS222 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-MEK1 in immunological assays. Activated MEK1 can be used in vitro to phosphorylate ERK protein. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Stabilizer: None

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MEK1 (MAP2K1 Produkte)

Synonyme: Mek1 Protein, CFC3 Protein, MAPKK1 Protein, MEK1 Protein, MKK1 Protein, PRKMK1 Protein, MEKK1 Protein, Prkmk1 Protein, mek-2 Protein, si:ch211-242m18.2 Protein, wu:fj56a12 Protein, wu:fj61b01 Protein, zgc:56557 Protein, ATMEK1 Protein, F20B18.180 Protein, F20B18_180 Protein, MAP kinase/ ERK kinase 1 Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 Protein, NMAPKK Protein, MEK Protein, mitogen activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 L homeolog Protein, MAP kinase/ ERK kinase 1 Protein, Map2k1 Protein, MAP2K1 Protein, map2k1.L Protein, map2k1 Protein, MEK1 Protein

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling