SNCA Protein (full length)

Produktnummer ABIN5651245

Produktdetails

Target: SNCA (Synuclein, alpha (SNCA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: full length

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Sequenz: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA

Spezifität: ~14.46 kDa

Aufreinigung: Ion-exchange Purified

Biological Activity Comment: Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer (SPR-323) seeded with 10 nM alpha synuclein protein PFF (SPR-324) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated an increased fluorescence intensity after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Certified >95% pure using SDS-PAGE analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: PBS pH 7.4

Lagerung: -80 °C

Antigendetails

Target: SNCA (Synuclein, alpha (SNCA))

Andere Bezeichnung: Alpha Synuclein (SNCA Produkte)

Synonyme: snca Protein, MGC64356 Protein, LOC619283 Protein, NACP Protein, alphaSYN Protein, PARK1 Protein, PARK4 Protein, PD1 Protein, synuclein alpha L homeolog Protein, alpha-synuclein Protein, synuclein alpha Protein, synuclein, alpha Protein, snca.L Protein, LOC619283 Protein, SNCA Protein, Snca Protein

Hintergrund: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

Gen-ID: 20617

NCBI Accession: NP_001035916

UniProt: O55042

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity