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DBR1 Protein (AA 228-544) (His tag)
DBR1
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 90 % by SDS - PAGE
SDS
Produktnummer ABIN5853256
Produktdetails
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Target
Alle DBR1 Proteine anzeigen
DBR1
(Debranching Enzyme Homolog 1 (DBR1))
Protein-Typ
Recombinant
Proteineigenschaft
AA 228-544
Spezies
Alle Spezies für DBR1 Proteine
Human
Quelle
Alle Expressionssysteme für DBR1 Proteine
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses DBR1 Protein ist gelabelt mit His tag.
Applikation
SDS-PAGE (SDS)
Sequenz
MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA
Reinheit
> 90 % by SDS - PAGE
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Alternativen
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Format
Liquid
Konzentration
0.5 mg/mL
Buffer
Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
Lagerung
4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Antigendetails
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Target
DBR1
(Debranching Enzyme Homolog 1 (DBR1))
Andere Bezeichnung
DBR1 (DBR1 Produkte )
Synonyme
AW018415 Protein, wu:fc08f08 Protein, zgc:63930 Protein, ARABIDOPSIS THALIANA DEBRANCHING ENZYME 1 Protein, ATDBR1 Protein, F28M20.40 Protein, F28M20_40 Protein, debranching enzyme 1 Protein, dbr1 Protein, dbr1-a Protein, dbr1-b Protein, debranching RNA lariats 1 Protein, debranching enzyme homolog 1 (S. cerevisiae) Protein, Lariat debranching enzyme Protein, debranching enzyme 1 Protein, RNA lariat debranching enzyme Dbr1 Protein, debranching RNA lariats 1 S homeolog Protein, debranching RNA lariats 1 L homeolog Protein, DBR1 Protein, Dbr1 Protein, dbr1 Protein, dbr-1 Protein, dbr1.S Protein, dbr1.L Protein
Hintergrund
DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. Recombinant human DBR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Molekulargewicht
38 kDa (340aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Accession
NP_057300
UniProt
Q9UK59
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