GOT1 Protein (AA 1-413) (His tag)
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- Target Alle GOT1 Proteine anzeigen
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-413
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Spezies
- Maus
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses GOT1 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ
- Reinheit
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 80 units/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
- Top Product
- Discover our top product GOT1 Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Liquid, In Phosphate buffered saline ( pH 7.4) containing 10 % glycerol, 1 mM DTT
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- Andere Bezeichnung
- Got1 (GOT1 Produkte)
- Synonyme
- ASTQTL1 Protein, GIG18 Protein, cAspAT Protein, cCAT Protein, AST Protein, ISSD Protein, NSD Protein, SD Protein, SIALIN Protein, SIASD Protein, SLD Protein, xr406 Protein, CASPAT Protein, ASPARTATE AMINOTRANSFERASE Protein, ATAAT Protein, MATERNAL EFFECT EMBRYO ARREST 17 Protein, MEE17 Protein, T26C19.9 Protein, T26C19_9 Protein, aspartate aminotransferase Protein, aatA Protein, 83.t00021 Protein, AI789014 Protein, Got-1 Protein, Aspat Protein, Gaspat Protein, AL022787 Protein, FABP-pm Protein, Got-2 Protein, mAspAT Protein, ASPATA Protein, glutamic-oxaloacetic transaminase 1 Protein, solute carrier family 17 member 5 Protein, glutamic-oxaloacetic transaminase 1 homeolog Protein, aspartate aminotransferase Protein, glutamic-oxaloacetic transaminase 1, soluble Protein, glutamic-oxaloacetic transaminase 2 Protein, glutamatic-oxaloacetic transaminase 2, mitochondrial Protein, GOT1 Protein, SLC17A5 Protein, got1.S Protein, AAT Protein, aspC Protein, EHI_006080 Protein, AST2 Protein, ast2 Protein, got1 Protein, Got1 Protein, GOT2 Protein, Got2 Protein
- Hintergrund
- Got1 also known as Glutamate oxaloacetate transaminase 1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant mouse Got1 fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 48.6kDa (436aa) confirmed by MALDI-TOF
- Pathways
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
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