FNDC5
(Fibronectin Type III Domain Containing 5 (FNDC5))
Biologische Aktivität
Active
Proteineigenschaft
AA 2-137
Spezies
Human
Quelle
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses FNDC5 Protein ist gelabelt mit His tag.
Applikation
Activity Assay (AcA), Cell Culture (CC)
Produktmerkmale
Tag location: N-terminal His Tag
Reinheit
> 90 %
Biological Activity Comment
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.