IL-2 Protein (AA 21-169) (His tag)
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- Target Alle IL-2 (IL2) Proteine anzeigen
- IL-2 (IL2) (Interleukin 2 (IL2))
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 21-169
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Spezies
- Maus
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses IL-2 Protein ist gelabelt mit His tag.
- Applikation
- Activity Assay (AcA), Cell Culture (CC)
- Produktmerkmale
- Tag location: N-terminal His Tag
- Reinheit
- > 97 %
- Biological Activity Comment
- Interleukin-2 (IL-2), the main lymphokine produced by activated CD4+ T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope was shown in Figure 1.
- Top Product
- Discover our top product IL2 Protein
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- Applikationshinweise
- Isoelectric Point: 4.9
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Konservierungsmittel
- Dithiothreitol (DTT), Other preservative, ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IL-2 (IL2) (Interleukin 2 (IL2))
- Abstract
- IL2 Produkte
- Synonyme
- IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein
- Hintergrund
- Alternative Names: TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
- Molekulargewicht
- 25kDa
- UniProt
- P04351
- Pathways
- JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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