IL17C Protein (AA 36-187) (His tag)
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- Target Alle IL17C Proteine anzeigen
- IL17C (Interleukin 17C (IL17C))
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 36-187
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses IL17C Protein ist gelabelt mit His tag.
- Applikation
- Cell Culture (CC), Activity Assay (AcA)
- Produktmerkmale
- Tag location: N-terminal His Tag
- Reinheit
- > 95 %
- Biological Activity Comment
- IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to plays a crucial role in innate immunity of the epithelium. IL17C binds to IL17RA with affinity. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17C with recombinant human IL17RA. Briefly, IL17C were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17C with IL17RA.
- Top Product
- Discover our top product IL17C Protein
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- Applikationshinweise
- Isoelectric Point: 8.6
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Konservierungsmittel
- Dithiothreitol (DTT), Other preservative, ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IL17C (Interleukin 17C (IL17C))
- Abstract
- IL17C Produkte
- Synonyme
- IL17C Protein, il17-3 Protein, CX2 Protein, IL-17C Protein, IL-21 Protein, interleukin 17C Protein, interleukin 17c Protein, IL17C Protein, il17c Protein, Il17c Protein
- Hintergrund
- Alternative Names: Cytokine CX2
- Molekulargewicht
- 19kDa
- UniProt
- Q9P0M4
- Pathways
- Cellular Response to Molecule of Bacterial Origin
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