Factor VII Protein (AA 315-433) (His tag)

Produktnummer ABIN6239863

Produktdetails

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Biologische Aktivität: Active

Proteineigenschaft: AA 315-433

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Factor VII Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 95 %

Biological Activity Comment: The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating FVII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as an interactor of FVII, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FVII and recombinant rat TF. Briefly, FVII were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TF-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FVII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of FVII and TF was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of FVII with TF.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Abstract: F7 Produkte

Synonyme: F7 Protein, wu:fb59f04 Protein, zgc:109870 Protein, SPCA Protein, AI114908 Protein, ATIII Protein, At-3 Protein, At3 Protein, LOC100223776 Protein, AI132620 Protein, Cf7 Protein, FVII Protein, mfVII Protein, f7 Protein, coagulation factor VII Protein, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Protein, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog Protein, F7 Protein, f7 Protein, Serpinc1 Protein, CpipJ_CPIJ009142 Protein, CpipJ_CPIJ010295 Protein, CpipJ_CPIJ020127 Protein, fa7 Protein, f7.S Protein

Hintergrund: Alternative Names: FVII, SPCA, proconvertin, Cothromboplastin, Serum Prothrombin Conversation Accelerator, Stable Factor, Eptacog alfa

Molekulargewicht: 14kDa

UniProt: Q8K3U6

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling