ALDH1A1
(Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
Biologische Aktivität
Active
Proteineigenschaft
AA 2-501
Spezies
Human
Quelle
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses ALDH1A1 Protein ist gelabelt mit His tag.
Applikation
Activity Assay (AcA), Cell Culture (CC)
Produktmerkmale
Tag location: N-terminal His Tag
Reinheit
> 95 %
Biological Activity Comment
ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ALDH1A1 with IL21.