CYP1B1
(Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))
Biologische Aktivität
Active
Proteineigenschaft
AA 374-516
Spezies
Human
Quelle
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses CYP1B1 Protein ist gelabelt mit His tag.
Applikation
Activity Assay (AcA), Cell Culture (CC)
Produktmerkmale
Tag location: N-terminal His Tag
Reinheit
> 97 %
Biological Activity Comment
Cytochrome P450 family 1 subfamily B member 1 (CYP1B1) is an enzyme, Which belongs to the cytochrome P450 superfamily. CYP1B1 can catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. Besides, Heat Shock Protein family B (small) member 2 (HSPb2) has been identified as an interactor of CYP1B1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1B1 and recombinant human HSPb2, CYP1B1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPb2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1B1 and HSPb2 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CYP1B1 with HSPb2.
CYP1B1
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA
Applikationshinweise
Isoelectric Point: 7.9
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Konservierungsmittel
Dithiothreitol (DTT), Other preservative, ProClin
Vorsichtsmaßnahmen
This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Target
CYP1B1
(Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))