IL-18 Protein (AA 37-193)
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- Target Alle IL-18 (IL18) Proteine anzeigen
- IL-18 (IL18) (Interleukin 18 (IL18))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 37-193
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- IL-18 (human) (rec.)
- Spezifität
- Human IL-18 (aa 37-193).
- Produktmerkmale
- Protein. Human IL-18 (aa 37-193). Source: E. coli. Endotoxin content: <0.06EU/μg purified protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in 1X PBS. Purity: >98 % (SDS-PAGE). Interleukin-18 (IL-18) is a costimulatory factor for production of interferon-gamma (IFN-gamma) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-gamma also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 have also been reported, such as enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF, IFN-gamma in T cells and enhancement of Fas ligand expression by Th1 cells.
- Reinheit
- >98 % (SDS-PAGE)
- Endotoxin-Niveau
- <0.06EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product IL18 Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Konzentration
- Lot specific
- Buffer
- Lyophilized from 0.2μm-filtered solution in 1X PBS.
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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- Target
- IL-18 (IL18) (Interleukin 18 (IL18))
- Andere Bezeichnung
- IL-18 (IL18 Produkte)
- Synonyme
- Igif Protein, Il-18 Protein, IL-18 Protein, IGIF Protein, ChIL-18 Protein, IL-1g Protein, IL1F4 Protein, IL18 Protein, interleukin 18 Protein, Il18 Protein, IL18 Protein
- Hintergrund
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Alternate Names/Synonyms: Interleukin-18, Interferon-gamma-inducing Factor, IGIF, IL-1gamma, IL1F4
Product Description: Interleukin-18 (IL-18) is a costimulatory factor for production of interferon-gamma (IFN-gamma) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-gamma also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 have also been reported, such as enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF, IFN-gamma in T cells and enhancement of Fas ligand expression by Th1 cells.
- Molekulargewicht
- ~18kDa
- NCBI Accession
- NP_001553
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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