IL1A Protein (AA 113-271)

Produktnummer ABIN6253323

Produktdetails

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 113-271

Spezies: Human

Quelle: HEK-293 Cells

Verwendungszweck: IL-1alpha (human) (rec.)

Spezifität: Human IL-1alpha (aa 113-271).

Produktmerkmale: Protein. Human IL-1alpha (aa 113-271). Source: HEK 293 cells. Endotoxin content: <0.01EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.001 ng/ml, corresponding to a specific activity of > 1 x 109units/mg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handhabung: Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Antigendetails

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1alpha (IL1A Produkte)

Synonyme: IL-1A Protein, IL1 Protein, IL1-ALPHA Protein, IL1F1 Protein, IL-6 Protein, Il-1a Protein, IL-1 alpha Protein, IL-1alpha Protein, L1A Protein, interleukin 1 alpha Protein, interleukin 6 Protein, IL1A Protein, IL6 Protein, Il1a Protein

Hintergrund:

Alternate Names/Synonyms: Interleukin-1alpha, IL-1F1, IL-1A

Product Description: The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

Molekulargewicht: ~19kDa (SDS-PAGE)

NCBI Accession: NP_000566

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints