NAMPT Protein (AA 1-491) (DYKDDDDK Tag)

Produktnummer ABIN6253488

Produktdetails

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-491

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses NAMPT Protein ist gelabelt mit DYKDDDDK Tag.

Verwendungszweck: Nampt (mouse) (rec.) (enzymatically active)

Spezifität: Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.

Kreuzreaktivität: Maus

Produktmerkmale: Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.

Reinheit: >90 % (SDS-PAGE)

Endotoxin-Niveau: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: 1μg to 5μg of Nampt (mouse) (rec.) (enzymatically active) show activity in a colorimetric Nampt assay.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Contains PBS.

Handhabung: After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Andere Bezeichnung: Nampt (NAMPT Produkte)

Hintergrund:

Visfatin, PBEF, Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase, NAmPRTase

Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column.

Molekulargewicht: ~55kDa (SDS-PAGE), ~100-110 kDa (Dimer) (SEC)

UniProt: Q99KQ4