GALT Protein (His tag)

Produktnummer ABIN6387110

Produktdetails

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GALT Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.5) containing 0.2 M NaCl, 10% glycerol.

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Andere Bezeichnung: GALT (GALT Produkte)

Hintergrund: GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Molekulargewicht: 45.9 kDa (403aa), confirmed by MALDI-TOF

NCBI Accession: NP_000146