GLO1 Protein (AA 1-184)
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- Target Alle GLO1 Proteine anzeigen
- GLO1 (Glyoxalase I (GLO1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-184
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH
- Reinheit
- > 90 % by SDS - PAGE
- Endotoxin-Niveau
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity: > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.
- Top Product
- Discover our top product GLO1 Protein
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Glyoxalase I (GLO1) (AA 2-184) protein (His tag)
GLO1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyoxalase I (GLO1) (AA 1-184) proteinGLO1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Glyoxalase I (GLO1) (AA 1-184) protein (GST tag)GLO1 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Glyoxalase I (GLO1) protein (His tag)GLO1 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Glyoxalase I (GLO1) (AA 1-184) (Active) protein (His tag)GLO1 Spezies: Maus Wirt: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS Active
Glyoxalase I (GLO1) protein (Myc-DYKDDDDK Tag)GLO1 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GLO1 (Glyoxalase I (GLO1))
- Andere Bezeichnung
- GLO1 (GLO1 Produkte)
- Hintergrund
- Lactoylglutathione lyase, also known as GLO1, belongs to the glyoxalaseIfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 20.7 kDa (184 aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_006699
- UniProt
- Q04760
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