PTH Protein (AA 32-115)

Produktnummer ABIN6387772

Produktdetails

Target: PTH (Parathyroid Hormone (PTH))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 32-115

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Sequenz: MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ

Reinheit: > 95 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 for this effect is less than or equal to 50 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In phosphate-buffered Saline(  pH 7.4) containing 2 mM EDTA

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PTH (Parathyroid Hormone (PTH))

Andere Bezeichnung: PTH (PTH Produkte)

Synonyme: PTH1 Protein, Pthp Protein, PTH-(1-84) Protein, Pth1 Protein, Pthr1 Protein, PTH Protein, parathyroid hormone Protein, parathyroid hormone S homeolog Protein, PTH Protein, Pth Protein, pth.S Protein

Substanzklasse: Hormone

Hintergrund: Human parathyroid hormone(hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques

Molekulargewicht: 9.55 kDa (85 aa), confirmed by MALDI-TOF

NCBI Accession: NP_000306

UniProt: P01270

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process